Det matematisk-naturvitenskapelige fakultet

Institutt for mikrobiologi

Produksjonsdato : 2001-01-23
Katalogtype: Fullformat.

1
PublKat: C11 (Rapport utgitt av institusjon, forening, o.a.)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Tittel:  Algal Virus Workshop : Abstracts - Program - Participiants : Bergen, N
         orway June 14 - 18, 1998
Red:     Bratbak, Gunnar
Red:     [et al.],
År:      1998
Side(r): 27 s.
Utgiver: Department of Microbiology, University of Bergen
URL:     http://www.fou.uib.no/fd/1998/f/414001/
Språk:   eng
PublID:  r98024533
StatKat: f

2
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Bratbak, Gunnar
         UiB, Institutt for mikrobiologi
Tittel:  Ecology of algal host-virus interactions
Tidskr:  Algal Virus Workshop
År:      1998
Side(r): s. 16
Utgiver: Department of Microbiology, University of Bergen
Konfer:  Algal Virus Workshop
Sted:    Bergen, 14.06-18.06
SamEng:  Virus and virus like particles have been observed in about 50 differe
         nt algal species but few algal-host-virus systems have been brought i
         nto culture and studied. The possible ecological significance of viru
         ses has been inferred from the presence of algal cells containing vir
         us like particles in natural communities, the presence of viruses in
         natural waters infecting specific algal populations and from studies
         of algal and virus population dynamics. Laboratory experiments with <
         EM>Phaeocystis pouchetii and the lytic virus PpV01 show that P. pouchetii is susceptible to virus infection in both exponent
         ial and stationary growth phase. Virus reproduction and cell lysis wa
         s not inhibited by nutrient (N or P) or light limitation, but inferio
         r growth conditio ns caused a decrease in burst size of >50%. Viral i
         nfection allowed primary production in the cells to continue through
         out most of the ly tic cycle. During lysis of the algal cells the ent
         ire algal biomass was converted to DOC, which was efficiently utilize
         d for bacterial bi omass production.
URL:     http://www.fou.uib.no/fd/1998/f/414001/
Språk:   eng
PublID:  r98024465
StatKat: f

3
PublKat: D01 (Invitert foredrag ved vit. konf. med publisert artikkel (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Bratbak, Gunnar
         UiB, Institutt for mikrobiologi
Medforf: Heldal, Mikal
         UiB, Institutt for mikrobiologi
Tittel:  Ecology of Phaeocystis host-virus interactions
Tidskr:  New Frontiers : Program & Abstracts
År:      1998
UtgSted: Halifax, Canada
Konfer:  Eight International Symposium on Microbial Ecology (ISME-8)
Sted:    Halifax, Canada, 09.08-14.08
SamEng:  The interaction between Phaeocystis pouchetii and the lytic
         virus PpV01 vas investigated in laboratory microcosmos. The
         experiment show thatP. pouchetiiis susceptible to virus
         infection in all stages of growth, and that nitrate, phosphate and
         light limitation of algal growth do not prevent virus reproduction
         and cell lysis. Inferior growth co nditions caused a decrease in
         burst size of >50% compared to more copious growth conditions.The
         lenght of the lytic cycle and the infectivity of the viruses was
         apparently not affected by the hosts growth conditions. Viral
         infection did not affect photosyntesis until near the onset of cell
         lysis. Release of dissolved organic car bon was low in non-infected
         cultures, while in infected cultures the entire algal biomass was
         converted to DOC. The DOC released during viral lysis was rapidly
         and efficiently utilized for bacterial growth.
Språk:   eng
PublID:  r99003184
StatKat: f

4
PublKat: A11 (Artikkel i internasjonalt vitenskapelig tidsskrift med referee)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Bratbak, Gunnar
         UiB, Institutt for mikrobiologi
Medforf: Jacobsen, Anita
         UiB, Institutt for fiskeri- og marinbiologi
Medforf: Heldal, Mikal
         UiB, Institutt for mikrobiologi
Tittel:  Viral lysis of Phaeocystis pouchetii and bacterial secondary productio
         n
Tidskr:  Aquatic Microbial Ecology
År:      1998-10-15
Volum:   16
Side(r): 11-16
Utgiver: Inter-research
Emneord: Phaeocystis pouchetii, Virus, DOC, Bacteria, Microbial food web
SamEng:  In this experimental study we have investigated the effect of viral i
         nfection on primary production and carbon flow in a phytoplankton - D
         OCbacteria food chain during viral lysis of the phytoplankton populat
         ion. The phytoplankter host-virus system used was Phaeocystis pouchet
         ii (Prymnesiophyceae) and the virus PpV01. Viral infection allowed pr
         imary production in the cells to continue throughout most of the lyti
         c cycle. In non-infected algal cultures, net production of DOC and ba
         cterial biomass was low and at the end of the experiment the DOC conc
         entration was 10-20%, and the bacterial biomass 0.5-4% of the algal c
         arbon biomass. The amount of DOC released during viral lysis of the a
         lgal cells imply that the entire algal biomass was converted to DOC.
         Growth of bacteria succeeding cell lysis and release of DOC in virus
         infected cultures demonstrated that the net effect of the virus infec
         tion was an efficient conversion of algal biomass into bacterial biom
         ass.
Språk:   eng
PublID:  r98030580
StatKat: f

5
PublKat: A11 (Artikkel i internasjonalt vitenskapelig tidsskrift med referee)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Bratbak, Gunnar
         UiB, Institutt for mikrobiologi
Medforf: Jacobsen, Anita
         UiB, Institutt for fiskeri- og marinbiologi
Medforf: Heldal, Mikal
         UiB, Institutt for mikrobiologi
Medforf: Nagasaki, Keizo
Medforf: Thingstad, Frede
         UiB, Institutt for mikrobiologi
Tittel:  Virus production in Phaeocystis pouchetii and its relation to host cel
         l growth and nutrition
Tidskr:  Aquatic Microbial Ecology
År:      1998-10-15
Volum:   16
Side(r): 1-9
Utgiver: Inter-Research
Emneord: Phaeocystis pouchetii, Virus, Nutrients, Growth condition
SamEng:  In this experimental study we have investigated how growth conditions
          and physiological status of the marine prymnesiophyte Phaeocystis po
         uchetii affects its interaction with the lytic virus PpV01. A simple
         mathematical model describing the infection, the lysis of algae and t
         he production of new viral particles was developed as an aid for anal
         yzing and understanding the interaction between host and virus. P. po
         uchetii was susceptible to virus infection in all stages of growth. N
         utrient or light limitation of algal growth did not inhibit viral rep
         roduction and cell lysis. Neither the infectivity of the progeny viru
         ses produced nor the length of the lytic cycle appeared to be affecte
         d by the host cells' growth condition. However, possible effects may
         have been obscured by low accuracy of infective virus counts or to lo
         w sampling frequency. The host cells' growth conditions did have a si
         gnificant impact on burst size. A maximum of 510 viruses produced per
          infected host cell was found in exponentially growing cultures, whil
         e low burst size (minimum 15) was found in stationary phase cultures,
          in nutrient depleted cultures and in light limited cultures.
Språk:   eng
PublID:  r98030582
StatKat: f

6
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Brussard, Corina
         UiB, Institutt for mikrobiologi
Medforf: Marie, Dominique
         Station Biologique de Rosscoff, France
Medforf: Thyrhaug, Runar
         UiB, Institutt for mikrobiologi
Medforf: Vaulot, Daniel
Medforf: Bratbak, Gunnar
         UiB, Institutt for mikrobiologi
Tittel:  Characterization of virus infected phytoplankton cells by FCN
Tidskr:  Algal Virus Workshop
År:      1998
Side(r): s. 13
Utgiver: Department of Microbiology, University of Bergen
Konfer:  Algal Virus Workshop
Sted:    Bergen, 14.06-18.06
SamEng:  The significance of viruses infecting phytoplankton cells has receive
         d much attention the last years. Algal viruses have been detected in
         different algal species and enumeration in natural waters suggests th
         at they play an important role in phytoplankton dynamics. Causing lys
         is of the algal cells, viruses will also indirectly affect the carbon
          and nutrient flow in the pelagic systems. However, until now not muc
         h is known on the cellular characteristics of viral infected phytopla
         nkton cells. We have used flow cytometry to analyse changes incellula
         r parameters during viral infection as compared to non-infected cells
          of two axenic phytoplankton species (Phaeocystis pouchetii
         and Micromonas pusilla). Diff erences in forward angle, side
          angle scatter and autofluorescence were observed for both algal spec
         ies. The use of fluorescent dyes furthermore yielded information on D
         NA content and viability which is not easily obtained with other meth
         ods. The use of these techniques in this field of research is new and
          provides detailed information for the study of the ecophysi ology of
          viral infected phytoplankton species.
URL:     http://www.fou.uib.no/fd/1998/f/414001/
Språk:   eng
PublID:  r98024464
StatKat: f

7
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Bødtker, Gunhild
         UiB, Institutt for mikrobiologi
Medforf: Polden Lillebø, Bente-Lise
         UiB, Institutt for mikrobiologi
Medforf: Thorbjørnsen, Bente
         UiB, Institutt for mikrobiologi
Medforf: Torsvik, Terje
         UiB, Institutt for mikrobiologi
Medforf: Thorstenson, Tore
Tittel:  Mikrobiologisk kontroll av vannsystemer på oljeplattformer i Nordsjøen
Tidskr:  Abstracts
År:      1998-02-19
Konfer:  Mikrobiologisk vintermøte
Sted:    Voss
SamNor:  Veksten av sulfatreduserende bakterier (SRB) i rørsystemer på oljepla
         ttformer overvåkes blant annet for å begrense korrosjon som skyldes m
         ikrobiell produksjon av hydrogensulfid (H2S). Populasjonen av SRB bes
         temmes ved telling med immunfluorescens (IF), og sulfatreduksjonsrate
         n måles radiologisk. IF målinger er hurtige og gjør det mulig å kvant
         ifisere en betydelig større andel av SRB enn det en kan gjøre med dyr
         king på tradisjonelle medier. Med disse metodene er det vist at spesi
         fikke SRB anrikes i vanninjeksjonssystemet som følge av vannbehandlin
         gen, og at det skjer endring i SRB populasjonen over tid. Resultatene
          har vært benyttet til å redusere det totale kjemikalie forbruket på
         plattformen uten at dette har ført til økt mikrobiell aktivitet. I de
         nne prosessen ble blant annet konsentrasjonen av biocid redusert til
         1/3 av det som opprinnelig ble benyttet. Ved ytterligere reduksjon av
          biocid dosering ble det observert økt SRB aktivitet samtidig som det
          ble registrert en økning i korrosjonsraten. Så langt har det imidler
         tid ikke vært mulig å påvise at økt korrosjon er en følge av redusert
          biocid eller økt SRB aktivitet.
Språk:   nor
PublID:  r99012514
LokalK:  Ns

8
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Bødtker, Gunhild
         UiB, Institutt for mikrobiologi
Medforf: Polden Lillebø, Bente-Lise
         UiB, Institutt for mikrobiologi
Medforf: Thorbjørnsen, Bente
         UiB, Institutt for mikrobiologi
Medforf: Torsvik, Terje
         UiB, Institutt for mikrobiologi
Medforf: Thorstenson, Tore
Tittel:  Monitoring microbial activity on oil platforms.
Tidskr:  Programs and abstracts
År:      1998-10-17
Konfer:  Multidisciplinary 30th anniversary meeting in Bergen, Norwegian
          biochemical society
Sted:    Bergen
SamEng:  The growth of sulphate-reducing bacteria (SRB) in oil platform water
         injection pipelines is monitored in order to reduce corrosion induced
          by microbial production of hydrogen sulphide (H2S). SRB is quantifie
         d by direct counting using immunoflourescent technique (IF), and by m
         easuring the rate of sulphate respiration using the isotope 35S. Comp
         ared to methods based on growth on traditional media, the IF method m
         akes it possible to quantify a larger portion of the SRB population.
         Results show that due to deaeration of the injected water, SRB are en
         riched in the injection system. In order to reduce the SRB activity t
         he water is routinely treated with biocids. Results of the monitoring
          have been used to reduce the use of biocides and hence the total amo
         unt of chemicals on the platforms. Reducing the biocid treatment by 1
         /3 did not affect the SRB activity. Reduction exceeding 1/3 of the or
         iginal level though, resulted in a higher SRB activity. There is also
          evidence of a shift in the SRB population during the monitoring peri
         od, possibly due to selection towards biocidtolerant/-resistant SRB.
         The latest results show higher corrosion rates, but so far it has not
          been possible to prove that this is a consequens of reduced biocid o
         r increased SRB activity.
Språk:   eng
PublID:  r99012513
LokalK:  Ns

9
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Castberg, Tonje
         UiB, Institutt for mikrobiologi
Medforf: Bratbak, Gunnar
         UiB, Institutt for mikrobiologi
Medforf: Enger, Øivind
         UiB, Institutt for mikrobiologi
Tittel:  A PCR approach to specific quantification of Phaeocytis virus
Tidskr:  [Abstracts from 6EMMS]
År:      1998
Side(r): s. 23
Konfer:  Sixth European Marine Microbiology Symposium (6EMMS)
Sted:    Stiges, Spain, 17.05-21.05
Språk:   eng
PublID:  r99003465
StatKat: f

10
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Castberg, Tonje
         UiB, Institutt for mikrobiologi
Medforf: Bratbak, Gunnar
         UiB, Institutt for mikrobiologi
Tittel:  Algevirus
Tidskr:  [Abstract]
År:      1998
Konfer:  Mikrobiologisk Vintermøte 98
Sted:    Voss, 19.02-22.02
Språk:   nor
PublID:  r99003999
StatKat: f
LokalK:  Ns

11
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Castberg, Tonje
         UiB, Institutt for mikrobiologi
Medforf: Enger, Øivind
         UiB, Institutt for mikrobiologi
Medforf: Bratbak, Gunnar
         UiB, Institutt for mikrobiologi
Tittel:  Detection of Phaeocytus-virus by PCR
Tidskr:  Algal Virus Workshop
År:      1998
Side(r): s. 12
Utgiver: Department of Microbiology, University of Bergen
UtgSted: Bergen, 14.06-18.06
Konfer:  Algal Virus Workshop
SamEng:  Current methods for detection and quantification of viruses are
         basedeither on titration, electron or epifluorescence microscopy.
         Titration requires an established host-virus system and
         time-consumingculturing, while microscopy lacks potential for
         specific detection. Ecological studies of algal viruses require
         reliable, specific techniques for detection and quantification. To
         study the ecology of Phaeocystis pouchetii - PpV host virus system
         weare working on a competitive PCR approach, applying an internal
         standard template that allows specific and sensitive detection of
         thevirus both in algal cultures and sea water samples. To develop a
         specific PCR for the Phaeocystis virus(PpV) we have used degenerate
         primers specific for phycodn aviridae, and sequenced the obtained
         PCRproduct to design specific primers for PpV. This approach also
         allowsus to compare the sequenced strains with other well described
         algal viruses. This highly versatile methodological approach may be
         appliedalso to other culturable vira and possibly also to vi ral sys
         tems not yet isolated.
URL:     http://www.fou.uib.no/fd/1998/f/414001/
Språk:   eng
PublID:  r98024463
StatKat: f
LokalK:  N

12
PublKat: A11 (Artikkel i internasjonalt vitenskapelig tidsskrift med referee)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Chatzinotas, Antonis
         ETH, Institute of Terrestrial Ecology, Soil Biology
Medforf: Sandaa, Ruth-Anne
         UiB, Institutt for mikrobiologi
Medforf: Schönhuber, Wilhelm
Medforf: Amann, Rudolf
Medforf: Daae, Frida Lise
         UiB, Institutt for mikrobiologi
Medforf: Torsvik, Vigdis
         UiB, Institutt for mikrobiologi
Medforf: Zeyer, Josef
         ETH, Institute of Terrestrial Ecology, Soil Biology
Medforf: Hahn, Dittmar
         ETH, Institute of Terrestrial Ecology, Soil Biology
Tittel:  Analysis of broad-scale differences in microbial community composition
          of two pristine forest soils.
Tidskr:  Systematic and Applied Microbiology
År:      1998
Volum:   21
Side(r): 579-587
Utgiver: Gustav Fischer Verlag
Emneord: DNA reassociation, in situ hybridization, % G+C content, probes, rRNA
Språk:   eng
PublID:  r99007052

13
PublKat: A11 (Artikkel i internasjonalt vitenskapelig tidsskrift med referee)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Drønen, Asbjørg Karine
         UiB, Institutt for mikrobiologi
Medforf: Torsvik, Vigdis
         UiB, Institutt for mikrobiologi
Medforf: Goksøyr, Jostein
         UiB, Institutt for mikrobiologi
Medforf: Top, Eva Marie
         University of Ghent, Belgia
Tittel:  Effect of mercury addition on plasmid incidence and gene mobilizing ca
         pasity in bulk soil
Tidskr:  FEMS Microbiology Ecology
År:      1998
Volum:   27
Side(r): 381-394
Utgiver: Elsevier
Emneord: Soil; mercury amendment; community shift; broad host-range plasmid
Språk:   eng
PublID:  r99007050

14
PublKat: C13 (Doktoravhandling)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Drønen, Asbjørg Karine
         UiB, Institutt for mikrobiologi
Tittel:  Plasmid mediated gene exchange in soil
Serie:   Dr.scient.
ISBN:    82-7788-064-2
År:      1998-09-18
Side(r): 1 b.
Utgiver: Universitetet i Bergen
Tilgang: Universitetsbiblioteket i Bergen - Byttekontoret
Emneord: plasmids, bacteria, gene exchange, conjugation, soil
SamEng:  http://www.fou.uib.no/drgrad/1998/414003/
URL:     http://www.uib.no/info/dr_grad/98/september/dronen/dronen.html
Språk:   eng
PublID:  r99001299
StatKat: a
LokalK:  N

15
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Eide, A.G.
Medforf: Mazengia, K.Y.
Medforf: Torsvik, Vigdis Lid
         UiB, Institutt for mikrobiologi
Medforf: Thorsvik, Berit Kristine
         UiB, Institutt for mikrobiologi
Medforf: Enger, Øivind
         UiB, Institutt for mikrobiologi
Medforf: Sandaa, Ruth-Anne
         UiB, Institutt for mikrobiologi
Tittel:  Spread of the plasmid pRAS1 in sea water and marine sediment -
         characterization of recipients [poster]
Tidskr:  [Abstracts from 6EMMS]
År:      1998
Konfer:  Sixth European Marine Microbiology Symposium (6EMMS)
Sted:    Stiges, Spain, 17.05-21.05
Språk:   eng
PublID:  r99003972
StatKat: f

16
PublKat: D05 (Uspesifisert poster med eller uten publisering (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Emmerhoff, Oddmund Johan
         UiB, Institutt for mikrobiologi
Medforf: Birkeland, Nils-Kåre
         UiB, Institutt for mikrobiologi
Tittel:  Chaperonins from the hyperthermophilic archaeon Archaeoglobus
         fulgidus
Tidskr:  Thermophiles '98
Red:     Prieur, Daniel
År:      1998-09
Side(r): 1 s.
Konfer:  Thermophiles '98
Sted:    Brest, Frankrike
Emneord: heat shock, chaperonin, archaeon
SamEng:  We have cloned and sequenced the genes encoding two chaperonin
         subunits (Cpn-a and Cpn-b), from Archaeoglobus fulgidus, a
         sulfate-reducing hyperthermophilic archaeon. The genes enc ode
         proteins of 545 amino acids with calculated Mr of 58977 and 59683.
         Both proteins have been identified in cytoplasmic fractions of A.
         fulgidus by western analysis using antibodiesraised against one of
         the subunits expressed in Escherichia coli, and by N-terminal amino
         acid sequencing of chaperonin complexes purified by
         immunoprecipitation. The chaperonin genes appear to be under heat
         shock regulation, as both proteins accumulate following temperature
         shift-up of growing A. fulgidus cells, implying a role of the
         chaperonin in thermoadaptation. Canonical Box A and Box B archaeal
         promoter sequences, as well as additional conserved putative signal
         sequences, are located upstream from the start codons. A phylogenetic
         analys is using all the available archaeal chaperonin sequences,
         suggests that the a and b subunits are results of late gene
         duplications that took place well after the establishment of the main
         archaeal evolutionary lines.
Språk:   eng
PublID:  r98028163
StatKat: f
LokalK:  s

17
PublKat: A11 (Artikkel i internasjonalt vitenskapelig tidsskrift med referee)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Emmerhoff, Oddmund Johan
         UiB, Institutt for mikrobiologi
Medforf: Klenk, Hans-Petter
Medforf: Birkeland, Nils-Kåre
         UiB, Institutt for mikrobiologi
Tittel:  Characterization and sequence comparison of temperature-regulated
         chaperonins from the hyperthermophilic archaeon Archaeoglobus
         fulgidus
Tidskr:  Gene
Red:     Bernardi, A
ISSN:    0378-1119
År:      1998-07-30
Volum:   215
Side(r): 431-438
Utgiver: Elsevier
Emneord: Archaea, heat shock, thermophiles, phylogeny
SamEng:  We have cloned and sequenced the genes encoding two chaperonin
         subunits (Cpn-a and Cpn-b), from Archaeoglobus fulgidus, a
         sulfate-reducing hyperthermophilic archaeon. The genes enc ode
         proteins of 545 amino acids with calculated Mr of 58977 and 59683.
         Both proteins have been identified in cytoplasmic fractions of A.
         fulgidus by western analysis using antibodiesraised against one of
         the subunits expressed in Escherichia coli, and by N-terminal amino
         acid sequencing of chaperonin complexes purified by
         immunoprecipitation. The chaperonin genes appear to be under heat
         shock regulation, as both proteins accumulate following temperature
         shift-up of growing A. fulgidus cells, implying a role of the
         chaperonin in thermoadaptation. Canonical Box A and Box B archaeal
         promoter sequences, as well as additional conserved putative signal
         sequences, are located upstream from the start codons. A phylogenetic
         analys is using all the available archaeal chaperonin sequences,
         suggests that the a and b subunits are results of late gene
         duplications that took place well after the establishment of the main
         archaeal evolutionary lines.
Språk:   eng
PublID:  r98028159
StatKat: f

18
PublKat: D05 (Uspesifisert poster med eller uten publisering (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Emmerhoff, Oddmund Johan
         UiB, Institutt for mikrobiologi
Medforf: Birkeland, Nils-Kaare
         UiB, Institutt for mikrobiologi
Tittel:  Characterization of Chaperonins form the hyperthermophilic archaeon Archaeoglobus fulgidus
År:      1998-01-22
Konfer:  Norsk Biokjemisk Selskabs Kontaktmøte
Sted:    Lillehammer
Språk:   eng
PublID:  r98030479
StatKat: f
LokalK:  Ns

19
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Enger, Øivind
         UiB, Institutt for mikrobiologi
Medforf: Sandaa, Ruth-Anne
         UiB, Institutt for mikrobiologi
Tittel:  Bacterial activity and diversity measured by differential-PCR
Tidskr:  [Abstract]
År:      1998
Konfer:  Mikrobiologisk Vintermøte 98
Sted:    Voss, 19.02-22.02
Språk:   nor
PublID:  r99004007
StatKat: f
LokalK:  Ns

20
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Enger, Øivind
         UiB, Institutt for mikrobiologi
Tittel:  Use of quantitative PCR fpr enumeration of the fish pathogen
         Aeromonas salmonivida in environmental samples
Tidskr:  [Abstract]
År:      1998
Konfer:  Mikrobiologisk Vintermøte 98
Sted:    Voss, 19.02-22.02
Språk:   eng
PublID:  r99004009
StatKat: f
LokalK:  Ns

21
PublKat: D04 (Uspesifisert foredrag med eller uten publisering (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Fagerbakke, Kjell Magne
         UiB, Institutt for mikrobiologi
Medforf: Norland, Svein
         UiB, Institutt for mikrobiologi
Medforf: Heldal, Mikal
         UiB, Institutt for mikrobiologi
Tittel:  Elemental composition in single native marine bacteria
Tidskr:  Örverufræðifélag Íslands, Örverur í hafinu
År:      1998-11-21
Konfer:  Nasjonalt møte i Islansk Forening for Mikrobiologi
Sted:    Reykjavik, Island
Emneord: XRMA, marin, bakterier, ratio
SamEng:  By X-ray microanalysis (XRMA) in the transmission electron microscope
          (TEM), it is possible to measure most of the important elements in s
         ingle bacteria. In bacterial communities sampled from coastal water,
         we find that indication of bacterial growth is easiest identified by
         the inorganic ion composition. Growing cells may be recognised by int
         ernal magnesium concentrations that is 10-20 times higher than outsid
         e (mean internal concentrations of 500-800 mM) or unbalanced internal
          molar Na Cl-1 ratio(<0.5). The range determined for mean bacterial c
         arbon per volume ratios was varying within an order of magnitude (30-
         200 fg mm-3) indicating that the water content in the bacteria are va
         riable. Low cellular N C-1 and P C-1 ratios of bacteria may indicate
         what is the limiting nutrient for natural bacterial communities. Acco
         rding to the Droop model there may be a correlation between these rat
         ios and growth rate, and by applying the Droop model to the XRMA resu
         lts we found typically that no single element limits all bacteria in
         the same marine sample. The overall molar ratio of 50:10:1 (C, N and
         P respectively) determined indicates that bacteria have relative high
         er content of N and P compared to microalgae. Multivariate statistics
          applied to XRMA results (ions excluded) from various marine samples,
          have shown that differences between samples may be detected, and thi
         s may correlate to nutrient availability. Within the bacterial divers
         ity present in the Scandinavian coastal water the element composition
          of bacteria seems to be more dependent on nutrient availabillity tha
         n on species diversity.
URL:     http://info.rfisk.is/users/hei/orv_fel.htm
Språk:   eng
PublID:  r98030435
StatKat: f
LokalK:  As

22
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Fagerbakke, Kjell Magne
         UiB, Institutt for mikrobiologi
Medforf: Norland, Svein
         UiB, Institutt for mikrobiologi
Medforf: Heldal, Mikal
         UiB, Institutt for mikrobiologi
Tittel:  Inorganic ions in marine bacteria
Tidskr:  [Abstract]
År:      1998
Konfer:  Mikrobiologisk Vintermøte 98
Sted:    Voss, 19.02-22.02
Språk:   eng
PublID:  r99004005
StatKat: f
LokalK:  Ns

23
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Fagerbakke, Kjell Magne
         UiB, Institutt for mikrobiologi
Medforf: Norland, Svein
         UiB, Institutt for mikrobiologi
Medforf: Heldal, Mikal
         UiB, Institutt for mikrobiologi
Tittel:  Inorganic ions in marine bacterioplanktona
Tidskr:  [Abstract]
År:      1998
Konfer:  The Scandinavian society for electron microscopy:
         50th Annual Meeting (SCANDEM-98)
Sted:    Espoo - Helsinki, Finland, 07.06-10.06
Språk:   eng
PublID:  r99004027
StatKat: f
LokalK:  As

24
PublKat: D04 (Uspesifisert foredrag med eller uten publisering (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Fagerbakke, Kjell Magne
         UiB, Institutt for mikrobiologi
Medforf: Norland, Svein
         UiB, Institutt for mikrobiologi
Medforf: Heldal, Mikal
         UiB, Institutt for mikrobiologi
Tittel:  X-ray microanalysis (XRMA): single cell analysis of biological signifi
         cant elements. CNP(OS) ratios and diffusible ions in micro-organisms.
Tidskr:  Ikkje publisert
År:      1998-10-23
Konfer:  Invitert gjesteforelesing, Universitetet i Århus
Sted:    Institutt for Mikrobiell Økologi
Emneord: XRMA, marine bacteria, magnesium, carbon
Språk:   eng
PublID:  r98027978
StatKat: f
LokalK:  As

25
PublKat: C15 (Hovedfagsoppgave)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Gausdal, Gro
Tittel:  Seterettet mutagenese og termostabilitet til isocitrat dehydrogenase f
         ra Archaeoglobus fulgidus
Serie:   cand.scient.-oppgave
År:      1998
Side(r): 169 s.
Utgiver: Universitetet i Bergen
Emneord: mikrobiologi
Språk:   nor
PublID:  r98025550
StatKat: f
LokalK:  N

26
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Heldal, Mikal
         UiB, Institutt for mikrobiologi
Medforf: Tuomi, P.
         Tvärminne Zoological Station, Finnish Environment Institute, H:ki
Medforf: Norland, Svein
         UiB, Institutt for mikrobiologi
Medforf: Bratbak, Gunnar
Tittel:  Isolates of marine bacteria: elemental content relates to growth
         conditions [poster]
Tidskr:  [Abstract]
År:      1998
Konfer:  Mikrobiologisk Vintermøte 98
Sted:    Voss, 19.02-22.02
Språk:   eng
PublID:  r99004024
StatKat: f
LokalK:  Ns

27
PublKat: C15 (Hovedfagsoppgave)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Hvoslef, Hilde
Tittel:  Ekspresjon og karakterisering av det termostabile isocitrat dehydrogen
          ase fra Archaeoglobus fulgidus
Serie:   cand.scient.-oppgave
År:      1998
Side(r): 92 bl.
Utgiver: Universitetet i Bergen
Emneord: mikrobiologi
Språk:   nor
PublID:  r98025551
StatKat: f
LokalK:  N

28
PublKat: C15 (Hovedfagsoppgave)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Høj, Lone
         UiB, Institutt for mikrobiologi
Tittel:  Metanogene arker i arktisk tundra - Populasjons-sammensetning i miljøe
         t og i anrikningskulturer med ulike karbonkilder
År:      1998-08
Side(r): 132 s.
Utgiver: Universitetet i Bergen
Tilgang: Institutt for mikrobiologi, Lone Høj
Emneord: Metanogene arker, arktisk tundra,
Språk:   nor
PublID:  r99001271
LokalK:  N

29
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Høj, Lone
         UiB, Institutt for mikrobiologi
Medforf: Torsvik, Vigdis
         UiB, Institutt for mikrobiologi
Tittel:  Metanogene bakterier i arktiske våtmarksområder
Tidskr:  Abstracts, Mikrobiologisk vintermøte 98
Red:     Utkilen, Hans
År:      1998-02-19
Konfer:  Mikrobiologisk vintermøte 98
Sted:    Voss
Emneord: metan, metanogene bakterier, metanogene arker, samfunns-analyser, DGG
         E, tundra
SamNor:  Metanogene bakterier i arktiske våtmarksområder Lone Høj og Vigdis To
         rsvik Institutt for mikrobiologi, Universitetet i Bergen Undersøkelse
         ne inngår i samarbeidsprosjektet "Effekter av klimatiske endringer på
          mikroorganismer involvert i produksjon og forbruk av drivhusgassen m
         etan". Målet er å øke forståelsen av mikrobiell omsetning av metan i
         arktisk tundra. Jordprøver ble tatt fra ulike lokaliteter på Svalbard
         . For to av prøvene ble det satt opp anrikningskulturer på ulike C-ki
         lder, disse ble dyrket ved 10OC. Samfunnsstrukturen av metanogene bak
         terier ble undersøkt ved å analysere DNA ekstrahert direkte fra jord
         og DNA fra anrikningskulturene. PCR-amplifiserte 16S rDNA sekvenser b
         le separert ved denaturerende gradient gel elektroforese (DGGE). Domi
         nerende bånd ble sekvensert for en tentativ identifisering av bakteri
         ene. Direkte ekstrahert DNA ga bånd med sekvenser som enten viste hom
         ologi med ikke-dyrkede Archaea eller liten homologi med sekvensene i
         databasen. Foreløpige resultater tyder på at typiske våtmarksområder
         på ulike steder av Svalbard domineres av de samme organismene. De fle
         ste av de dominerende organismene vokser i anrikninger på acetat, for
         mat og H2+CO2. Noen vokser i tillegg på metanol og TMA. Organismer so
         m dominerer på flere lokaliteter vil bli førsøkt isolert for fysiolog
         isk og genetisk karakterisering. Effekter av endring i temperatur og
         fuktighet på den metanogene samfunnsstrukturen vil bli studert i felt
          og i mikrokosmosforsøk.
Språk:   nor
PublID:  r99012507
LokalK:  Ns

30
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Høj, Lone
         UiB, Institutt for mikrobiologi
Medforf: Rusten, Marte
         UiB, Institutt for mikrobiologi
Medforf: Torsvik, Vigdis
         UiB, Institutt for mikrobiologi
Tittel:  Methanogenic archaea in Arctic tundra investigated by enrichment cultu
         res and molecular methods
Tidskr:  Programme & Abstracts Norwegian Biochemical Society, Bergen
          Section, Multidiciplinary 30th Anniversary Meeting in Berg
         en
Red:     Blystad, Frøydis
Red:     Vedeler, Anni
År:      1998-10-17
Side(r): 1 s.
Konfer:  Norwegian Biochemical  Society, Bergen Section, Multidiciplinar
         y 30th Anniversary Meeting in Bergen
Sted:    Bergen
Emneord: methane, methanogens, DGGE, nested PCR, community analysis
SamEng:  METHANOGENIC ARCHAEA IN ARCTIC TUNDRA INVESTIGATED BY A COMBINATION O
         F ENRICHMENT CULTURES AND MOLECULAR METHODS Lone Høj, Marte Rusten an
         d Vigdis Torsvik Department of Microbiology, University of Bergen Dom
         inating types of methanogenic archaea in soil and sediment samples fr
         om Spitsbergen were determined by using a combination of molecular me
         thods and enrichment cultures. Total-DNA was isolated directly, and e
         nrichment cultures on five different carbon sources were made anaerob
         ically and incubated at 10§C. DNA coding for the hypervariable V3 reg
         ion of 16S rRNA was amplified from both total-DNA and harvested cells
         , using a nested PCR protocol with primers specific for Archaea. The
         amplified fragments were separated by denaturing gradient gel electro
         phoresis (DGGE). Dominating and unique bands were excised, reamplifie
         d and sequenced for a tentative identification of the organisms. By t
         his approach the archaeal community structure at different sites coul
         d be compared, as well as changes in composition of the methanogenic
         population in the different enrichments. Results from this study indi
         cated that draining conditions were important for determining the dev
         elopment of the archaeal community structure. The only methanogenic a
         rchaea detected in total-DNA was related to acetate-specialists in th
         e genus Methanosaeta, and the sequence was only detected in soils wit
         h poor draining. However, the enrichment cultures showed that in addi
         tion to the Methanosaeta-related organism, hydrogenotrophic and methy
         lotrophic methanogenic archaea could also grow at 10§C when substrate
          was added, indicating that they were substrate-limited in the soil.
Språk:   eng
PublID:  r99012509
LokalK:  Ns

31
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Høj, Lone
         UiB, Institutt for mikrobiologi
Medforf: Torsvik, Vigdis
         UiB, Institutt for mikrobiologi
Tittel:  Methanogenic bacteria in Arctic wetland areas.
Tidskr:  New Frontiers: Program & Abstracts
År:      1998-08-09
Konfer:  The 8th International Symposium on Microbial Ecology (ISME-8)
Sted:    Halifax, Canada
Emneord: methane, methanogenic bacteria, methanogenic archaea, community struc
         ture, DGGE, tundra,
SamEng:  Høj, L., and Torsvik, V., Department of Microbiology, University of B
         ergen, N-5020 Bergen, Norway. METHANOGENIC BACTERIA IN ARCTIC WETLAND
          AREAS. The methanogenic community structure in wetland areas on Sval
         bard was studied by a combination of new molecular methods and enrich
         ment cultures. Total DNA was extracted directly from soil and sedimen
         t samples. Enrichment cultures on five different carbon sources were
         made for two sampling sites, and grown at 10OC. Total DNA and bacteri
         al pellets were both subject to PCR amplification using Archaea-speci
         fic primers, and the amplified 16S rDNA sequences were separated by d
         enaturing gradient gel electrophoresis (DGGE). Dominating and/or uniq
         ue bands were sequenced for a tentative identification of the bacteri
         a. The same DGGE pattern were found for all typical wetland sites in
         this study, while areas that were likely to dry up during summer had
         a different community structure. One site had a combination of the tw
         o patterns. Most of the organisms dominating in the environment grew
         in enrichment cultures on acetate, formate and H2+CO2. Some could als
         o grow on methanol and trimethylamine (TMA), but opportunistic bacter
         ia tended to proliferate on these carbon sources. Sequences from the
         directly extracted DNA showed homology to uncultured Archaea or low h
         omology to sequences in the database.
Språk:   eng
PublID:  r99012504
LokalK:  s

32
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Høj, Lone
         UiB, Institutt for mikrobiologi
Medforf: Torsvik, Vigdis
         UiB, Institutt for mikrobiologi
Tittel:  Methanogenic bacteria in Arctic wetland areas
Red:     McDonald, Ian
Red:     Bourne, David
År:      1998-04-16
Konfer:  4th Molecular Microbial Ecology Group (MMEG) meeting
Sted:    Warwick, England
Emneord: methane, methanogenic bacteria, methanogenic archaea, community struc
         ture, DGGE, tundra
SamEng:  Methanogenic bacteria in arctic wetland areas. Lone Høj and Vigdis To
         rsvik Department of microbiology, University of Bergen DNA was extrac
         ted directly from soil from different sites on Svalbard. The methanog
         enic community structure on the different sites were analysed by sepa
         rating PCR amplified 16S rDNA sequences by denaturing gradient gel el
         ectrophoresis (DGGE). Dominating bands were sequenced and compared wi
         th sequences in a database. Enrichment cultures on different carbon s
         ources were made with soil from two of the sites. The cultures were a
         nalysed by the same methods as the directly extracted DNA. Isolation
         of organisms that dominated on several sites will be attempted for ph
         ysiological and genetical characterisation. Impact of physical parame
         ters like temperature and humidity will be studied in field and in mi
         crocosm.
Språk:   eng
PublID:  r99012505
LokalK:  s

33
PublKat: A11 (Artikkel i internasjonalt vitenskapelig tidsskrift med referee)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Jensen, Sigmund
Medforf: Øvreås, Lise
         UiB, Institutt for mikrobiologi
Medforf: Daae, Frida Lise
         UiB, Institutt for mikrobiologi
Medforf: Torsvik, Vigdis
         UiB, Institutt for mikrobiologi
Tittel:  Diversity in methane enrichments from agricultural soil revealed by DG
         GE separation of PCR amplified 16S rDNA fragments
Tidskr:  FEMS Microbiology Ecology
År:      1998
Volum:   25
Side(r): 1-10
Utgiver: Elsevier Science
Emneord: soil, enrichment, methanotroph, denaturing gradient gel electrophores
         is, phylogeny
Språk:   eng
PublID:  r99001259

34
PublKat: A12 (Artikkel i internasjonalt vitenskapelig tidsskrift uten referee)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Knutsen, Gjert
         UiB, Institutt for mikrobiologi
Medforf: Hansen, Kjersti
         UiB, Institutt for mikrobiologi
Tittel:  From sea to test: Search for bioactive substances from marine
         microalgae and cyanobacteria.
Tidskr:  Marine microorganisms for industry. Proceedings of the
         meeting held in Brest-France on the 17-19th of September
         1997.
Red:     Le Gal, Yves
Red:     Muller-Feuga, Arnaud
ISSN:    0761-3962
ISBN:    2-905434-94-5
År:      1998
Side(r): 169-178
Utgiver: IFREMER
Emneord: Bioactive substances, marine microalgae, marine cyanobacteria
SamEng:  Marine microorganisms are coming more and more into focus as new
         sources for bioactive compounds. The cyanobacteria and microalgae of
         the cold Norw waters and adjacen oceans are an untapped resource for
         such compounds. We have therefore developed a set of methods for
         exploring the potential of this source by large scale screening for
         bioactive compounds from these organisms.The methods extend from the
         mass isolation of organisms, via production of biomass and extracts
         ready for screening, as well as optimization. The system consists of
         procedures that we have thoroughly tested and evaluated for
         effectiveness and reproducibility. Extracts of 75 organisms have been
         tested in a few bioassays in different systems for bioact ivity, and
         with interesting results.
Språk:   eng
PublID:  r99000392
StatKat: f

35
PublKat: D04 (Uspesifisert foredrag med eller uten publisering (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Larsen, Øivind
         UiB, Institutt for mikrobiologi
Medforf: Urtegaard, Victoria
         UiB, Institutt for mikrobiologi
Medforf: Lien, Torleiv
         UiB, Institutt for mikrobiologi
Medforf: Birkeland, Nils Kåre
         UiB, Institutt for mikrobiologi
Tittel:  Detection of sulfate- and sulfite-reducing bacteria using dissimilator
         y sulfite reductase as a target gene
Tidskr:  Norwegian Biochemical Society, Bergen Section
År:      1998-10-17
Side(r): 1 s. s.
Konfer:  Multidisciplinary 30th Anniversary Meeting
Sted:    Bergen
SamEng:  Sulfate-reducing prokaryotes carry out anaerobic respiration by utili
         zation of sulfate as a therminal electron acceptor and by this produc
         e the highly toxic and corrosive gass hydrogensulfide. These microorg
         anisms constitute a large physiological group of anaerobes including
         representatives from the delta-subgroup of proteobacteria, the Gram-p
         ositive genus Desulfotomaculum, the deep-branching thermophilic bacte
         rial genera Thermodesulfobacterium and Thermodesulfovibrio, and the h
         yperthermophilic archaeal genus Archaeoglobus. Thus, although the abi
         lity of sulfate respiration is present in widely seperated phylogenet
         ic groups, it´s distribution appears to be confined to a limited numb
         er of main groups. Dissimilatory sulfite reductase (Dsr), which catal
         yses the therminal step in the reduction of sulfate to sulfide throug
         h a six-electron transfer, has been characterized from representative
         s of all these main groups. [...] Similar enzymes to Dsr have also be
         en identified in sulfite-reducing strains of Chlostridium and Salmone
         lla typhimurium, and in the sulfur-oxidicing phototroph Chromatium vi
         nosum, and in the hyperthermophilic thiosulfate-reducing crenarchaeot
         e Pyrobaculum islandicum. Dsr is also remotely related to assimilator
         y sulfite and nitrate reductases from eukaryotes and bacteria. Initia
         l sequence comparisons of Dsr have reviled high sequence similarity b
         etween all of the dsr-genes so far sequenced. Thus, this makes Dsr a
         good candidate to monitor sulfate- and sulfite-reducing bacteria in t
         he environment.
Språk:   eng
PublID:  r99009041
LokalK:  Ns

36
PublKat: D05 (Uspesifisert poster med eller uten publisering (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Larsen, Øivind
         UiB, Institutt for mikrobiologi
Medforf: Lien, Torleiv
         UiB, Institutt for mikrobiologi
Medforf: Birkeland, Nils Kåre
         UiB, Institutt for mikrobiologi
Tittel:  Dissimilatory sulfite reductase from Archaeoglobus profundus and Desul
         fotomaculum thermocisternum: phylogenetic and structural implication f
         rom gene sequences
Tidskr:  Thermophiles ´98
År:      1998-09-06
Side(r): 1 s.
Konfer:  Thermophiles '98
Sted:    Brest, France
SamEng:  The genes encoding the a and b subunits of dissimilatory sulfite redu
         ctase, dsrAB, from the hyperthermophilic archaeon Archaeoglobus profu
         ndus and the thermophilic Gram-positive bacterium Desulfotomaculum th
         ermocisternum were cloned and sequenced. The dsrAB genes are contiguo
         us, and most probably comprise an operon also including a dsrD homolo
         gue; a conserved gene of unknown function located downstream of dsrAB
          in all four sulfate reducers so far sequenced. Sequence comparison c
         onfirms that dissimilatory sulfite reductase, Dsr, is a highly conser
         ved enzyme. A phylogenetic analysis using the available Dsr sequences
         , including Dsr-like proteins from non-sulfate reducers, suggests a p
         aralogous origin of the a and b subunits. Furthermore, the Dsr from s
         ulfate reducers forms a separate cluster, with Dsr from the bacterial
          sulfate reducers Desulfotomaculum thermocisternum and Desulfovibrio
         vulgaris branching together, next to Dsr from Archaeoglobus profundus
          and Archaeoglobus fulgidus. Based on an alignment with the assimilat
         ory sulfite reductase from Escherichia coli the amino acid residues i
         nvolved in binding of sulfite, siroheme and [Fe4S4]-clusters have bee
         n tentatively identified, which is consistent with the binding of 2 s
         irohemes and 4 [Fe4S4]-clusters per a2b2 structure. The evolution of
         Dsr and the structural basis for the binding of substrate and cofacto
         rs have been discussed.
Språk:   eng
PublID:  r99009042
LokalK:  s

37
PublKat: D05 (Uspesifisert poster med eller uten publisering (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Larsen, Øivind
         UiB, Institutt for mikrobiologi
Medforf: Urtegaard, Victoria B.
         UiB, Institutt for mikrobiologi
Medforf: Lien, Torleiv
         UiB, Institutt for mikrobiologi
Medforf: Birkeland, Nils Kåre
         UiB, Institutt for mikrobiologi
Tittel:  Dissimilatory sulfite reductase from sulfate-reducing prokaryotes isol
         ated from North Sea oil wells
År:      1998-02-12
Side(r): 1 s. s.
Konfer:  Norsk mikrobiologisk selskaps vintermøte
Sted:    Voss
SamEng:  Dissimilatory sulfite reductase is a key enzyme in the dissimilatory
         reduction of sulfate to sulfide. It has been found in all sulfate-red
         ucing prokaryotes thus far characterised. Karkhoff-Schweizer et al. h
         ave showed that the enzyme is highly conserved (1). We have tried to
         isolate dissimilatory sulfite reductase from both mesophilic and ther
         mophilic sulfate-reducing prokaryotes using conserved primers in PCR
         reactions. Sequence analysis reveals remarkable conservation of gene
         arrangement and sequence between all gene analysed. This makes dissim
         ilatory sulfite reductase a putative gene for detection of sulfate-re
         ducing prokaryotes.  1. Karkhoff-Schweizer, R. R., D. P. W. Huber, an
         d G. Voordouw. 1995. Conservation of the gene for dissimilatory sulfi
         te reductase from Desulfovibrio vulgaris and Archaeoglobus fulgidus a
         llows their detection by PCR.
Språk:   eng
PublID:  r99009045
LokalK:  Ns

38
PublKat: A11 (Artikkel i internasjonalt vitenskapelig tidsskrift med referee)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Lien, Torleiv
         UiB, Institutt for mikrobiologi
Medforf: Madsen, Marit
         UiB, Institutt for mikrobiologi
Medforf: Steen, Ida Helene
         UiB, Institutt for mikrobiologi
Medforf: Gjerdevik, Kjersti
         UiB, Institutt for mikrobiologi
Tittel:  Desulfobulbus rhabdoformis sp. nov., a sulfate reducer from a water-oi
         l separation system
Tidskr:  International Journal of Systematic Bacteriology (IJSB)
År:      1998
Volum:   48
Hefte:   2
Side(r): 469-474
Utgiver: Society for General Microbiology, Great Britain
Emneord: Desulfobulbus rhabdoformis sp. nov., sulfate reducer, 16S rRNA gene s
         equence, oilfield
Språk:   eng
PublID:  r98021734
StatKat: f

39
PublKat: D04 (Uspesifisert foredrag med eller uten publisering (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Lien, Torleiv
         UiB, Institutt for mikrobiologi
Tittel:   Invitert sesjonsforedrag: "Termofile mikroorganismer fra oljereservoa
         r i Nordsjøen"
Tidskr:  Foredraget er ikke publisert
År:      1998-02-21
UtgSted: Park hotel, Voss
Konfer:  Mikrobiologisk Vintermøte
Språk:   nor
PublID:  r98008512
StatKat: f
LokalK:  Ns

40
PublKat: D04 (Uspesifisert foredrag med eller uten publisering (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Lien, Torleiv
         UiB, Institutt for mikrobiologi
Tittel:  Mikrobiell aktivitet ved olje- og gassproduksjon i Nordsjøen.
År:      1998-03-19
UtgSted: Hotell Neptun
Konfer:  Årsmøte i Bergens avd. for Norsk korrosjon forening. Invitert
         foredragsholder
Språk:   nor
PublID:  r98021736
StatKat: f
LokalK:  Ns

41
PublKat: A11 (Artikkel i internasjonalt vitenskapelig tidsskrift med referee)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Lien, Torleiv
         UiB, Institutt for mikrobiologi
Medforf: Madsen, Marit
         UiB, Institutt for mikrobiologi
Medforf: Rainey, Fred A.
Medforf: Birkeland, Nils Kåre
         UiB, Institutt for mikrobiologi
Tittel:  Petrotoga mobilis sp. nov., from a North Sea oil-production well
Tidskr:  International Journal of Systematic Bacteriology
År:      1998-07
Volum:   48
Hefte:   3
Side(r): 1007-1013
Utgiver: Society for General Microbiology
Emneord: Petrotoga mobilis sp. nov., thermophile, oilfield water
Språk:   eng
PublID:  r98024482
StatKat: f

42
PublKat: A11 (Artikkel i internasjonalt vitenskapelig tidsskrift med referee)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Lien, Torleiv
         UiB, Institutt for mikrobiologi
Medforf: Madsen, Marit
         UiB, Institutt for mikrobiologi
Medforf: Birkeland, Nils-Kåre
         UiB, Institutt for mikrobiologi
Medforf: Rainey, Fred , Lousiana State,
         UiB, Institutt for mikrobiologi
Tittel:  Petrotoga mobilis sp. nov., from a North Sea oil-production well
Tidskr:  International Journal of Systematic Bacteriology
År:      1998
Volum:   48
Side(r): 1007-1014
Utgiver: Society for General Microbiology, Great Britain
Emneord: Petrotoga mobilis sp.nov., thermophile, oilfield water
Språk:   eng
PublID:  r98026216
StatKat: f

43
PublKat: A11 (Artikkel i internasjonalt vitenskapelig tidsskrift med referee)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Malin, G.
         Univ E Anglia, Sch Environm Sci, Norwich, England
Medforf: Wilson, W.H.
Medforf: Bratbak, Gunnar
         UiB, Institutt for mikrobiologi
Medforf: Liss, P.S.
Medforf: Mann, N.H.
Tittel:  Elevated production of dimethylsulfide resulting from viral infection
         of cultures of Phaeocystis pouchetii
Tidskr:  Limnology and Oceanography
ISSN:    0024-3590
År:      1998
Volum:   43
Side(r): 1389-1393
SamEng:  The effects of viral lysis on dimethyl sulfide (DMS) and particulate
         and dissolved dimethylsulfoniopropionate (DMSP) concentrations were
         studied in batch cultures of Phaeocystis pouchetii infected with a
         strain-specific viral isolate. After 20 h, DMS concentration had
         increased by fourfold over the levels observed in the control
         cultures, and this increased to eightfold 45 h after addition of
         viral particles. The results suggest that viral lysis of
         DMSP-containing phytoplankton cells should be considered alongside
         autolysis, grazing, and bacterial activity as a process that can
         influence DMS concentrations in seawater. Further research will be
         necessary to determine the importance of viruses in the
         biogeochemical DMS cycle in the natural environment.
Språk:   eng
PublID:  r99003169
StatKat: f

44
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Malin, G.
         Univ E Anglia, Sch Environm Sci, Norwich, England
Medforf: Wilson, W.H.
Medforf: Bratbak, Gunnar
         UiB, Institutt for mikrobiologi
Medforf: Liss, P.S.
Medforf: Mann, N.H.
Tittel:  Elevated production of dimethylsulfide resulting from viral infection
         of cultures of Phaeocystis pouchetii
År:      1998
Konfer:  Algal Virus Workshop
Sted:    Bergen, 15.06-18.06
PublID:  r99004058
StatKat: f
LokalK:  Ns

45
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Marie, Dominique
         Station Biologique de Roscoff, France
Medforf: Brussard, Corina
         UiB, Institutt for mikrobiologi
Medforf: Thyrhaug, Runar
         UiB, Institutt for mikrobiologi
Medforf: Bratbak, Gunnar
         UiB, Institutt for mikrobiologi
Medforf: Vaulot, Daniel
         Station Biologique de Roscoff, France
Tittel:  Enumeration of viruses in marine samples by flow cytometry [poster].
Tidskr:  Algal Virus Workshop
År:      1998
Side(r): s. 16
Utgiver: Department of Microbiology, University of Bergen
UtgSted: Bergen, 14.06-18.06
Konfer:  Algal Virus Workshop
SamNor:  [2] FCM was also applied to the analysis of natural samples from
         three different oceanic regions ranging from mesothropic to
         oligotrophic conditions, and, after staining with SYBR-I, we found
         different populations that have a lower green fluorescence from
         bacteria. We found virus concentration, about 10 fold higher than
         bacteria ranging from 0.2 X 107 ml-1 in coastal
         waters. We discriminate at least two virus-like populations (V-I and
         V-II) in marine assemblages. We observed that 20% of the V-I and 100%
         ofthe V-II-like viruses can pass through 0.2m-pore-size filters.
         Viral counts obtained on natural samples by EFM were lower than those
         performed by FCM, due to the difficulty to clearlydifferentiate
         virus-like particles by microscopy. The analysis of a depth profile
         in the Mediterrian Sea revealed that the abundance of virus particles
         displayed the same trndth an ba cteriaand autotrophic cells. FCM
         should prove very helpful for studies of virus infection in cultures
         and to understand better the structure and dynamic of virus
         populations in oceanic waters.
SamEng:  [1] During the last two decades, numereous effort have beeen invested
         in developing methods for detecting, recognozing and enumerating
         different microorganisms such as bacteria,algae, protozoa and viruses
         that are present in acuatic ecosystems. Flow cytometry (FCM) has been
         routinely used for the enumeration of the different populations
         composing the phytoplanktonic and heterotrophic bacterial
         communities. We have now begun to apply FCM to enumerate viruses in
         seawater, both in cultured and natural samples after staining with
         the nuclei c acid-specific dye SYBR Green I. Although FCM does not
         allow the identification and location of viruses associated with host
         cells, its major advantage resides in the possibility toanalyze
         quickly a large number of viral particles, therefore providing highly
         statistically significant data. The efficiency of FCM was checked
         during an infection experiment on Phaeocystis pouchetii.
         Virus counts obtained by FCM were highly correlated with those
         obtained by epifluorescence microscopy (EFM) or transmission elsctron
         microscopy (TEM). F CM counts were about 30% higher than those
         obtained by EFM and differences up to 15% were obtained with TEM.
URL:     http://www.fou.uib.no/fd/1998/f/414001/
Språk:   eng
PublID:  r98024530
StatKat: f

46
PublKat: A11 (Artikkel i internasjonalt vitenskapelig tidsskrift med referee)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Mostajir, B.
         Inst Natl Rech Sci Oceanol, Rimouski, Canada
Medforf: Fagerbakke, Kjell Magne
         UiB, Institutt for mikrobiologi
Medforf: Heldal, Mikal
         UiB, Institutt for mikrobiologi
Medforf: Thingstad, T. Frede
         UiB, Institutt for mikrobiologi
Tittel:  Elemental composition of individual pico- and nano-sized marine
         detrital particles in the northwestern Mediterranean Sea
Tidskr:  Oceanologica Acta
ISSN:    0399-1784
År:      1998
Volum:   21
Hefte:   4
Side(r): 589-596
Emneord: detritus, elemental composition, tem, x-ray, mediterranean
SamEng:  The elemental composition of individual < 10 m detrital particles
         from Mediterranean surface waters was analysed using a Transmission
         Electron Microscope (TEM) equipped withanenergy dispersive X-Ray
         microanalyser. Results show that carbon and phosphorus content per
         detritus volume are much higher in pico-detrital particles < 2 m
         (42 kg C m-3 and 1 kg P m-3) than in 5-10 m
         detrital particles (20 kg C m-3 and 0.1 kg P m-3). The
         C:N:P atomicratios for different sized fractions of the detrital pa
         rticl es were found to be 82:10:1 for < 2 m particles, 120:29:1 for
         2-5 m particles and 308:37:1 for 5-10 m particles. The average
         ratio forall size classes of detrital particles(< 10 m) was
         132:23:1. The differences in elementary compositions of the detrital
         particles studied here suggest that the different size fractions
         probably have di fferent orig ins. The role and origins of < 10 m
         detrital particles within the biogeochemical cycles are discussed.
Språk:   eng
PublID:  r99003142
StatKat: f

47
PublKat: A11 (Artikkel i internasjonalt vitenskapelig tidsskrift med referee)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Mostajir, Behzad
Medforf: Fagerbakke, Kjell Magne
         UiB, Institutt for mikrobiologi
Medforf: Heldal, Mikal
         UiB, Institutt for mikrobiologi
Medforf: Thingstad, T Frede
         UiB, Institutt for mikrobiologi
Medforf: Rassoulzadegan, Fereidoun
Tittel:  Elemental composition of individual pico- and nano- sized marine detri
         tal particles in the northwestern Medeteranian Sea.
Tidskr:  Oceanologica Acta
År:      1998-12
Volum:   21
Hefte:   6
Side(r): 589-596
Utgiver: Elsivier, Paris
Emneord: detritus, elemental composition, TEM, X-ray, Mediterranean
SamEng:  The elemental composition of individual <10 µm detrital particles fro
         m Mediterranean surface waters was analyzed using a Transmission Elec
         tron Microscope (TEM) equipped with an energy dispersive X-Ray microa
         nalyser. Results show that carbon and phosphorus contents per detritu
         s volume are much higher in pico-detrital particles (42 kg C m-3 and
         1 kg P m-3) than in 5-10 µm detrital particles (20 kg C m-3 and 0.1 k
         g P m-3). The C : N : P atomic ratios for different size fractions of
          the detrital particles were found to be 82 : 10 : 1 for < 2 µm parti
         cles, 120 : 29 : 1 for 2-5 µm particles and 308 : 37 : 1 for 5-10 µm
         particles. The average of all size classes of detrital particles <10
         µm was 132 : 23 : 1. The differences in elemental compositions of the
          detrital particles studied here suggest that the different size frac
         tions probably have different origin. The roles and origins of 10 µm
         detrital particles within the biogeochemical cycles are discussed.
URL:     http://www.elsevier.nl:80/inca/publications/store/6/0/0/7/8/7/index.h
Språk:   eng
PublID:  r98030443
StatKat: f

48
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Myhr, Siri
         UiB, Institutt for mikrobiologi
Medforf: Lillebø, Bente-Lise Polden
         UiB, Institutt for mikrobiologi
Medforf: Bødtker, Gunhild
         UiB, Institutt for mikrobiologi
Medforf: Torsvik, Terje
         UiB, Institutt for mikrobiologi
Medforf: Sunde, Egil
Medforf: Beeder, Janiche
Tittel:  Control of microbial H2S production in oil reservoirs by ni
         trate
Tidskr:  Programme and Abstracts. Multidisciplinary 30th Anniversary
          Meeting in Bergen. Norwegian Biochemical Society.
Red:     Brokstad, Karl
År:      1998-10-17
Konfer:  Multidisciplinary 30th Anniversary Meeting in Bergen. Norwegian
          Biochemical Society.
Sted:    Bergen
Emneord: sulfide, nitrate, SRB, DGGE, oil reservoir
SamEng:  Sulfate reducing bacteria (SRB) are present in oil reservoirs, produc
         ing H2S, a toxic and corrosive gas. Competition with other anaerobic
         bacteria, as nitrate reducing bacteria (NRB), is proposed to reduce H
         2S production. We have built an oil reservoir model column in order t
         o investigate the effect of nitrate addition on H2S production. Injec
         tion of 0,5 mM nitrate for 75-80 days lead to a complete inhibition o
         f sulfide production. SRB were probably inhibited by nitrite, produce
         d by NRB, and an increased redox potential due to a chemical oxidatio
         n of sulfide by nitrite. Polyclonal antibodies were developed against
          pure cultures of SRB and NRB and used for immunofluorescent counting
          of SRB and NRB before and after nitrate injection. SRB dominated the
          anaerobic zone of the column before nitrate addition and only a slig
         ht increase was observed in the NRB population after nitrate addition
         , excluding substrate competition as a major mechanism for H2S elimin
         ation. SRB survived for 1-3 months in an inactive state. Then a drama
         tic reduction in population size was detected (min. 60 ). This chang
         e was also evident by PCR/DGGE (Denaturant Gradient Gel Electrophores
         is) analysis. When nitrate was removed, sulfide production was detect
         ed after 2-3 months, and reached previous levels after 5-6 months. Du
         ring this period the SRB population was re-built and again dominated
         the DGGE patterns. Isolated strains of SRB and NRB were characterised
         . Based on phenotypic and phylogenetic data we suggest that two new g
         enera of SRB and NRB should be established. Proposed names for the ty
         pe strains are Desulfostipes sapovorans and Denitrovibrio acetophilus
         .
Språk:   eng
PublID:  r99012510
LokalK:  Ns

49
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Myhr, Siri
         UiB, Institutt for mikrobiologi
Medforf: Lillebø, Bente-Lise Polden
         UiB, Institutt for mikrobiologi
Medforf: Torsvik, Terje
         UiB, Institutt for mikrobiologi
Medforf: Sunde, Egil
Medforf: Bjørnestad, Eva
Medforf: Beeder, Janiche
Medforf: Granli, Tom
Tittel:  Control of reservoir souring by nitrate injection. Results from an oil
          reservoir model system.
Tidskr:  Abstracts fra Mikrobiologisk Vintermøte 98
Red:     Utkilen, Hans
År:      1998-02-19
Side(r): 1 s.
Konfer:  Mikrobiologisk Vintermøte
Sted:    Voss
Emneord: Reservoir souring, sulfide, nitrate, SRB, NRB
SamEng:  Reservoir souring, the production of H2S in oil reservoirs by sulfate
          reducing bacteria (SRB), causes operational and economical problems
         for the o industry. H2S is a toxic and corrosive gas and must be remo
         ved from the oil before transportation and processing. In this study
         we have built a model system of an oil reservoir and shown that injec
         tion of small amounts of nitrate can inhibit microbial H2S production
         : A continous injection of 0,5 mM nitrate for 75 days lead to a compl
         ete inhibition of the H2S production. The effect of nitrate was due t
         o stimulation of a different group of anaerobic bacteria, the nitrate
          reducing bacteria (NRB). SRB and NRB were counted by fluorescent ant
         ibodies at different sites in the column. Only a slight increase in t
         he NRB fraction was detected after nitrate addition, excluding subtra
         te competition between SRB and NRB as a major mechanism for H2S elimi
         nation.The most probable mechanism is NO2poisoning of the SRB, the NO
         2- being produced during nitrate reduction in NRB. NO2- also leads to
          an elevated redox potensial in the column, making a hostile environm
         ent for SRB. The diversity of bacteria in the column was investigated
          by PCR amplification of 16S rDNA sequences and separation of the seq
         uences by Denaturing Gradient Gel Electrophoresis. The DNA-bands were
          sequenced for a tentative identification of the bacteria. The result
         s showed, in agreement with the immunofluorescent counting, that the
         SRB population dominated the anaerobic part of the column both before
          and after nitrate addition. The effect of NO3-/NO2- on SRB was bacte
         riostatic, rather than bacteriocidal. H2S production was inhibited, b
         ut the SRB survived for a long period in an inactive state.
Språk:   eng
PublID:  r99012502
LokalK:  Ns

50
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Myhr, Siri
         UiB, Institutt for mikrobiologi
Medforf: Lillebø, Bente-Lise Polden
         UiB, Institutt for mikrobiologi
Medforf: Torsvik, Terje
         UiB, Institutt for mikrobiologi
Medforf: Sunde, Egil
Medforf: Bjørnestad, Eva
Medforf: Beeder, Janiche
Medforf: Hægh, Thor
Tittel:  Effect of nitrate injection on H2S production and microbial
          community composition in an oil reservoir model column.
Tidskr:  Kørber Symposium on Molecular and Microsensor Studies of Mi
         crobial communities. Program and Abstracts.
Red:     Jørgensen, Bo Barker
År:      1998-09-07
Konfer:  Kørber Symposium on Molecular and Microsensor Studies of Microb
         ial communities
Sted:    Bremen, Tyskland
Emneord: sulfide, SRB, nitrate, DGGE, community analysis
SamEng:  Sulfate reducing bacteria (SRB) are present in oil reservoirs, produc
         ing H2S, a toxic and corrosive gas. Competition with other anaerobic
         bacteria, as nitrate reducing bacteria (NRB) is proposed to reduce H2
         S production. In this study we have built an oil reservoir model syst
         em and shown that a continous injection of 0,5 mM nitrate for 75 days
          lead to a complete inhibition of the H2S production. Polyclonal anti
         bodies were produced against the pure cultures of SRB and NRB and use
         d for immunofluorescent counting of SRB and NRB in the column before
         and after nitrate addition. Only a slight increase in the NRB fractio
         n was detected after nitrate addition, excluding substrate competitio
         n between SRB and NRB as a major mechanism for H2S elimination.The mo
         st probable mechanism is NO2- poisoning of the SRB, the NO2- being pr
         oduced during nitrate reduction in NRB. NO2- also leads to an elevate
         d redox potensial in the column, making a hostile environment for SRB
         . The diversity of bacteria in the column was investigated by PCR amp
         lification of 16S rDNA sequences and separation of the sequences by D
         enaturing Gradient Gel Electrophoresis (DGGE). The DNA-bands were seq
         uenced for a tentative identification of the bacteria. The results sh
         owed, in agreement with the immunofluorescent counting, that the SRB
         population dominated the anaerobic part of the column both before and
          after nitrate addition. H2S production was inhibited by nitrate, but
          the SRB survived for a long period in an inactive state.
Språk:   eng
PublID:  r99012508
LokalK:  s

51
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Myhr, Siri
         UiB, Institutt for mikrobiologi
Medforf: Lillebø, Bente-Lise Polden
         UiB, Institutt for mikrobiologi
Medforf: Torsvik, Terje
         UiB, Institutt for mikrobiologi
Medforf: Sunde, Egil
Medforf: Bjørnestad, Eva
Medforf: Beeder, Janiche
Medforf: Granli, Tom
Tittel:  Inhibition of microbial hydrogen sulfide production by small amounts o
         f nitrate. Results from an oil reservoir model system.
Tidskr:  New Frontiers. Program and Abstracts. Eigth International S
         ymposium on Microbial Ecology (ISME-8)
Red:     Bell, Colin
Red:     Lewis, Trudy
År:      1998-08-09
Konfer:  8th International Symposium on Microbial Ecology (ISME-8)
Sted:    Halifax, Canada
Emneord: sulfide, nitrate, reservoir souring, SRB, DGGE
SamEng:  In this study we have built a model system of an oil reservoir and sh
         own that injection of 0,5 mM nitrate for 75 days leads to a complete
         inhibitio the H2S production. SRB and NRB were counted by
         fluorescent antibodies at different sites in the column. Only a sligh
         t increase in the NRB fraction was detected after nitrate addition, e
         xcluding substrate competition between SRB and NRB as a major mechani
         sm for H2S elimination.The most probable mechanism is NO2- poisoning of the SRB, the NO2-
          being produced during nitrate reduction in NRB. NO2
         - also leads to an increased redox potensial in the column
         , making a hostile environment for SRB. The diversity of bacteria in
         the column was investigated by PCR/DGGE/sequencing. The results showe
         d, in agreement with the immuno-fluorescent counting, that the SRB po
         pulation dominated the anaerobic part of the column both before and a
         fter nitrate addition. H2S production was inhibited by nit
         rate, but SRB survived for a long period in an inactive state.
Språk:   eng
PublID:  r99012506
LokalK:  s

52
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Myhr, Siri
         UiB, Institutt for mikrobiologi
Medforf: Torsvik, Terje
         UiB, Institutt for mikrobiologi
Medforf: Lillebø, Bente-Lise Polden
         UiB, Institutt for mikrobiologi
Medforf: Sunde, Egil
Medforf: Bjørnestad, Eva
Medforf: Beeder, Janiche
Medforf: Hægh, Thor
Tittel:  Small amounts of nitrate inhibit microbial sulfide production in an oi
         l reservoir model system.
Tidskr:  The 9th International Oil Field Chemicals Symposium
Red:     Lundeby, Gunn
År:      1998-03-22
Side(r): 10 s.
Konfer:  The 9th International Oil Field Chemicals Symposium
Sted:    Geilo
Emneord: Reservoir souring, sulfide, nitrate, SRB, nitrate reducing bacteria
SamEng:  Reservoir souring, the production of H2S in oil reservoirs by sulfate
          reducing bacteria (SRB), causes operational and economical problems
         for the o industry. H2S is a toxic and corrosive gas and must be remo
         ved from the oil before transportation and processing. In this study
         we built a model system of an oil reservoir and showed that a contino
         us injection of 0,5 mM nitrate for 75 days lead to a complete inhibit
         ion of the H2S production. The effect of nitrate was due to stimulati
         on of a different group of anaerobic bacteria, the nitrate reducing b
         acteria (NRB). SRB and NRB were counted by fluorescent antibodies at
         different sites in the column. Only a slight increase in the NRB frac
         tion was detected after nitrate addition, excluding subtrate competit
         ion between SRB and NRB as a major mechanism for H2S elimination.The
         most probable mechanism is NO2poisoning of the SRB, the NO2- being pr
         oduced during nitrate reduction in NRB. NO2- also leads to an elevate
         d redox potensial in the column, making a hostile environment for SRB
         . The diversity of bacteria in the column was investigated by PCR amp
         lification of 16S rDNA sequences and separation of the sequences by D
         enaturing Gradient Gel Electrophoresis. The DNA-bands were sequenced
         for a tentative identification of the bacteria. The results showed, i
         n agreement with the immunofluorescent counting, that the SRB populat
         ion dominated the anaerobic part of the column both before and after
         nitrate addition.H2S production was inhibited by nitrate, but the SRB
          survived for a long period in an inactive state. The low concentrati
         on of nitrate needed to inhibit SRB activity makes it a suitable agen
         t for reducing reservoir souring.
Språk:   eng
PublID:  r99012503
LokalK:  s

53
PublKat: A17 (Artikkel i populærvitenskapelig tidsskrift)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Nesje, Anne Marie Skriveseth
Medforf: Heldal, Mikal
         UiB, Institutt for mikrobiologi
Tittel:  Ei utvandrar-soge frå Tysnes
Tidskr:  Sunnhordland : Årbok frå Sunnhordland Folkemuseum og Sogela
         g
ISSN:    0333-0958
År:      1998
Volum:   79
Side(r): 66-76
Språk:   nno
PublID:  r99003172
StatKat: f

54
PublKat: C11 (Rapport utgitt av institusjon, forening, o.a.)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Patel, Sonal
         UiB, Institutt for mikrobiologi
Medforf: Torsvik, Vigdis
         UiB, Institutt for mikrobiologi
Medforf: Goksøyr, Jostein
         UiB, Institutt for mikrobiologi
Tittel:  Utbredelse av antibiotikaresistens i bakterier fra det ytre miljø
År:      1998
Side(r): 39 s.
Utgiver: Statens forurensningstiulsyn (SFT)
Tilgang: Statens forurensningstilsyn (SFT)
Emneord: antibiotikaresistens, bakterier, ytre miljø, jord, vann, sedimenter,
         kloakk
Språk:   nor
PublID:  r99001269
LokalK:  N

55
PublKat: D01 (Invitert foredrag ved vit. konf. med publisert artikkel (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Rassoulzadegan, Fereidouin
         Station Zoologique, Villefranche-s-Mer, Frankrike
Medforf: Thingstad, T. Frede
         UiB, Institutt for mikrobiologi
Tittel:  Changes in microbial size structure induced by silicate addition to
         mesocosms
År:      1998-02
Utgiver: American Society for Limnology and Oceanography
Konfer:  ASLO Ocean Sciences meeting
Sted:    San Diego
Språk:   eng
PublID:  r99004048
StatKat: f
LokalK:  s

56
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Rassoulzadegan, Fereidouin
         Station Zoologique, Villefranche-s-Mer, Frankrike
Medforf: Thingstad, T. Frede
         UiB, Institutt for mikrobiologi
Tittel:  Marine ecosystem - Food web structure as a function of nutrients
År:      1998-10
Konfer:  MATER meeting
Sted:    Rhodos
Språk:   eng
PublID:  r99004052
StatKat: f
LokalK:  s

57
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Sandaa, Ruth-Anne
         UiB, Institutt for mikrobiologi
Medforf: Daae, Frida Lise
         UiB, Institutt for mikrobiologi
Medforf: Catberg, Tonje
         UiB, Institutt for mikrobiologi
Medforf: Enger, Øivind
         UiB, Institutt for mikrobiologi
Medforf: Torsvik, Vigdis Lid
         UiB, Institutt for mikrobiologi
Tittel:  Changes im the microbiological community structure and diversity in
         metal contaminated soils
Tidskr:  [Abstract]
År:      1998
Konfer:  Mikrobiologisk Vintermøte 98
Sted:    Voss, 19.02-22.02
Språk:   eng
PublID:  r99003994
StatKat: f

58
PublKat: A11 (Artikkel i internasjonalt vitenskapelig tidsskrift med referee)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Sandaa, Ruth-Anne
         UiB, Institutt for mikrobiologi
Medforf: Enger, Øivind
         UiB, Institutt for mikrobiologi
Medforf: Torsvik, Vigdis
         UiB, Institutt for mikrobiologi
Tittel:  Rapid method for fluorometric quantification of DNA in soil
Tidskr:  Soil Biology and Biochemistry
År:      1998
Volum:   30
Hefte:   2
Side(r): 265-268
Utgiver: Pergamon, Elsevier
Emneord: DNA, quantification, soil
Språk:   eng
PublID:  r99001254

59
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Sandaa, Ruth-Anne
         UiB, Institutt for mikrobiologi
Medforf: Thorsen, Berit Kristine
         UiB, Institutt for mikrobiologi
Medforf: Mazengia, Kibret Timer
Medforf: Eide, Anne Grete
Medforf: Torsvik, Vigdis Lid
         UiB, Institutt for mikrobiologi
Medforf: Enger, Øivind
         UiB, Institutt for mikrobiologi
Tittel:  Transfer of the naturally occuring plasmid pRAS1 affects the microbial
         diversity in marine sediments
Tidskr:  [Abstracts from 6EMMS]
År:      1998
Side(r): s. 41
Konfer:  Sixth European Marine Microbiology Symposium (6EMMS)
Sted:    Stiges, Spain, 17.05-21.05
Språk:   eng
PublID:  r99003474
StatKat: f

60
PublKat: C13 (Doktoravhandling)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Steen, Ida Helene
         UiB, Institutt for mikrobiologi
Tittel:  Isocitrate dehydrogenase from the sulfate reducers Archaeoglobus fulgi
         dus and Desulfobacter vibrioformis:Properties, Primary structure, and
         Phylogeny
ISBN:    82-7761-008-4
År:      1998-03-27
Side(r): 1 b.
Utgiver: Universitetet i Bergen
Tilgang: Universitetsbiblioteket i Bergen, Byttekontoret
Emneord: mikrobiologi
SamEng:  http://www.fou.uib.no/drgrad/1998/414002/
URL:     http://www.uib.no/info/dr_grad/98/mars/steen/steen.html
Språk:   eng
PublID:  r98021731
StatKat: f
LokalK:  N

61
PublKat: A11 (Artikkel i internasjonalt vitenskapelig tidsskrift med referee)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Steen, Ida Helene
         UiB, Institutt for mikrobiologi
Medforf: Madsen, Marit Steine
         UiB, Institutt for mikrobiologi
Medforf: Birkeland, Nils-Kaare
         UiB, Institutt for mikrobiologi
Medforf: Lien, Torleiv
         UiB, Institutt for mikrobiologi
Tittel:  Purification and characterization of a monomeric isocitrate dehydrogen
         ase from the sulfate-reducing bacterium Desulfobacter vibrioformis and
          demonstration of the presence of a monomeric enzyme in other bacteria
Tidskr:  FEMS Microbiol. Letters
År:      1998-02
Volum:   160
Side(r): 469-474
Språk:   eng
PublID:  r99005034

62
PublKat: A11 (Artikkel i internasjonalt vitenskapelig tidsskrift med referee)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Thingstad, T. Frede
         UiB, Institutt for mikrobiologi
Tittel:  A theoretical approach to structuring mechanisms in the pelagic food
         web
Tidskr:  Hydrobiologica
ISSN:    0018-8158
År:      1998
Volum:   363
Side(r): 59-72
SamEng:  In the literature there is a commonly used idealized concept of the
         food web structure in the pelagic photic zone food web, based to a
         large extent on size dependent relationships. An outline is here
         given of how the elementary size-related physical laws of diffusion
         and sinking, combined with the assumption of predators being size
         selective in their choice of prey, give a theoretical foundation for
         this type of structure. It is shown how such a theoretical fundament
         makes it possible to relate a broad specter of phenomena w ithin one
         generic and consistent framework. Phenomena such as Hutchinson's and
         Goldman's paradoxes, the influence of nutrients and water column
         stability on the balance between microbial and classical food webs,
         bacterial carbon consumption, new production and export of DOC and
         POC to the aphotic zone, eutrophication and diversity, can all be
         approached from this perspective. By including host-specific viruses,
         this approach gives a hierarchical structure to the control of
         diversity with nutrient content controlling the maximum size of the
         photic zone community, size selectivity of predators regulating how
         the nutrient is distributed between size-groups of osmotrophic and
         phagotrophic organisms, and viral host specificity regulating how the
         nutrients within a size group is distributed between host groups. I
         also briefly discuss how some biological strategies may be successful
         by not conforming to the normal rules of such a framework. Analyzing
         the behavior of these idealized systems is thus claimed to facilitate
         our understanding of the behavior of complex natural food webs.
Språk:   eng
PublID:  r99003126
StatKat: f

63
PublKat: D01 (Invitert foredrag ved vit. konf. med publisert artikkel (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Thingstad, T. Frede
         UiB, Institutt for mikrobiologi
Medforf: Havskum, H.
Medforf: Kaas, H.
Medforf: Lefevre, D.
Medforf: Nielsen, T.G.
Medforf: Riemann, B.
Medforf: Williams, P.J.
Tittel:  Bacteria-protist interactions and organic matter degradation under
         P-limitation: enclosure experiment and a simple model
År:      1998-02
Utgiver: American Society for Limnology and Oceanography
Konfer:  ASLO Ocean Sciences meeting
Sted:    San Diego
Språk:   eng
PublID:  r99004112
StatKat: f
LokalK:  s

64
PublKat: D01 (Invitert foredrag ved vit. konf. med publisert artikkel (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Thingstad, T. Frede
         UiB, Institutt for mikrobiologi
Tittel:  Bacterial degradation of DOC, how much can we learn from simple steady
         -state descriptions
Tidskr:  New Frontiers : Program & Abstracts
År:      1998
UtgSted: Halifax, Canada
Konfer:  Eight International Symposium on Microbial Ecology (ISME-8)
Sted:    Halifax, Canada, 09.08-14.08
Språk:   eng
PublID:  r99004045
StatKat: f

65
PublKat: D01 (Invitert foredrag ved vit. konf. med publisert artikkel (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Thingstad, T. Frede
         UiB, Institutt for mikrobiologi
Medforf: Rassoulzadegan, Fereidouin
         Station Zoologique, Villefranche-s-Mer, Frankrike
Tittel:  Life between Skylla and Charybdis; microbes in a sea of competition
         and predation
Tidskr:  New Frontiers : Program & Abstracts
År:      1998
UtgSted: Halifax, Canada
Konfer:  Eight International Symposium on Microbial Ecology (ISME-8)
Sted:    Halifax, Canada, 09.08-14.08
Språk:   eng
PublID:  r99004041
StatKat: f

66
PublKat: D01 (Invitert foredrag ved vit. konf. med publisert artikkel (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Thingstad, T. Frede
         UiB, Institutt for mikrobiologi
Medforf: Fonnes, G.-A.
         UiB, Institutt for mikrobiologi
Medforf: Marassé, C.
Medforf: Gasol, J.M.
         Institute Ciencies del Mar, Barcelona, Spain
Medforf: Sala, M.M.
         Institute Ciencies del Mar, Barcelona, Spain
Medforf: Peters, F.
         Institute Ciencies del Mar, Barcelona, Spain
Medforf: Vidal, M.
         Institute Ciencies del Mar, Barcelona, Spain
Medforf: Zweifel, U.L.
         National Environmental Research Institute, Roskilde, Denmark
Medforf: Rassoulzadegan, Fereidouin
         Station Zoologique, Villefranche-s-Mer, Frankrike
Medforf: Fuhrman, J.
         University of California, Los Angeles, USA
Tittel:  Microbial affinity for orthophophate studied in microcosms with natura
         l plankton communities from western Mediterranean
År:      1998-02
Konfer:  6'th European Marine Microbiology Symposium
Sted:    Stiges, 17.05-22-05
Språk:   eng
PublID:  r99004113
StatKat: f
LokalK:  s

67
PublKat: A11 (Artikkel i internasjonalt vitenskapelig tidsskrift med referee)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Thingstad, T. Frede
         UiB, Institutt for mikrobiologi
Medforf: Zweifel, Ulla Li
         National Environment inst. Roskilde, Denmark
Medforf: Rassoulzadegan, Fereidoun
         Station Zoologique, Villefranche-sur-Mer, France
Tittel:  P limitation of heterotrophic bacteria and phytoplankton in the northw
         est                                      Mediterranean
Tidskr:  Limnology and Oceanography
ISSN:    0024-3590
År:      1998
Volum:   43
Hefte:   1
Side(r): 88-94
SamEng:  Surface-water microbial populations were investigated in the northwes
         t Mediterranean for possible indicators of     phosphate deficiency a
         nd limitation. Low phosphorus availability was suggested by shore tur
         nover time (min, observed     0.68 h), high-alkaline phosphatase acti
         vity (V-max = 28 nM hydrolyzed h(-1)), subsaturation of phosphate upt
         ake     (2.6-9% of V-max), and high-pulse uptake capacity of added or
         thophosphate. Based on high pulse uptake capacity and     subsaturate
         d uptake in both the >1-mu m and in the 0.2-1-mu m size fractions, P
         deficiency is suggested for both     phytoplankton and heterotrophic
         bacteria. P limitation of heterotrophic bacteria was also supported b
         y fast positive     responses after phosphate addition in both thymid
         ine incorporation in whole-water samples and increased bacterial cell
              numbers in predator-free water. No effects were found after addi
         tion of carbon or nitrogen sources alone. Combined     with other pub
         lished evidence, we suggest that the growth rates of not
         only phytoplankton, but also of heterotrophic     bacteria, are P lim
         ited in this environment in summer. The finding has important implica
         tions for the dynamics of     accumulation of dissolved organic carbo
         n in the photic zone and thus for the carbon cycle of oceans.
Språk:   eng
PublID:  r99003133
StatKat: f

68
PublKat: D01 (Invitert foredrag ved vit. konf. med publisert artikkel (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Thingstad, T. Frede
         UiB, Institutt for mikrobiologi
Tittel:  Steady state models of the bicrobial food web. A useful tool in
         exploration and management of Mediterranan surface waters?
År:      1998
Konfer:  35'th CIESM meeting
Sted:    Dubrovnik, 01.06-06.06
PublID:  r99004047
StatKat: f
LokalK:  s

69
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Thorsen, Berit Kristine
         UiB, Institutt for mikrobiologi
Medforf: Sandaa, Ruth-Anne
         UiB, Institutt for mikrobiologi
Medforf: Mazengia, Kibret Yimer
Medforf: Eide, Anne Grete
Medforf: Torsvik, Vigdis
         UiB, Institutt for mikrobiologi
Medforf: Enger, Øivind
         UiB, Institutt for mikrobiologi
Tittel:  Transfer of the naturally occuring plasmid pRAS1 affects the
         microbiological diversity in marine sediments [poster]
Tidskr:  [Abstract]
År:      1998
Konfer:  Mikrobiologisk Vintermøte 98
Sted:    Voss, 19.02-22.02
Språk:   eng
PublID:  r99004019
StatKat: f
LokalK:  Ns

70
PublKat: C15 (Hovedfagsoppgave)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Thyrhaug, Runar
Tittel:  Virusinfeksjon i Phaeocystis poucheti - infeksjonsforløpet
         karakterisert ved en-celle metoder
Serie:   cand.scient.-oppgave
År:      1998
Utgiver: Universitetet i Bergen
Emneord: mikrobiologi
SamNor:  Konsentrasjonen ac P. pouchetii ble kraftig redusert i løpet
         av 56 timer etter tilsetting av Phaeocystis pouchetii
         virus-01 (PpV-01) samtidig som konsentrasjonen av PpV-01 økte flere
         ganger sitt opprinnelige nivå. I ikke-aksenisk kultur økte også bakte
         riekonsentrasjonen markant. Det nukleinsyrespesifikke fargestoffet SY
         BRGreen-I (Molecular probes) ble benyttet i kombinasjon med floecytom
         etri til å kvantitivt bestemme konsentrasjonen av PpV-01. Flowcytomet
         ri kunne også dektere endringer i lavvinkel- (FSC) og vidvinkelspredn
         ing (SSC) og rød fluorescens per celle under infeksjonsforløpet. Gjen
         nomsnitlig signal per celle med hensyn på FSC, SSC og rød fluorescens
          var forskjellig i kontrollkultur og infisert kultur. DNA-innholdet p
         er celle økte utover i infeksjonsforløpet, målt ved dlowcytometri og
         billedanalyse. Ved å benytte flowcytometri og cellsyklusanalyse kunne
          fraksjonen virusinfiserte celler estimeres. Burst size ble beregnet
         på grunnlag av endringer i alge- og viruskonsentrasjoner, og ved å
         telle antallet synlige cirus ved transmisjons elektromikroskopi. Kons
         entrasjonen av ekstracellulære polyskkarider i infisert ikke-aksenisk
          kultur økte sterkt mellom 40 og 56 timer etter tilsetting av virus.
Språk:   nor
PublID:  r99003174
StatKat: f
LokalK:  N

71
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Torsvik, T
         UiB, Institutt for mikrobiologi
Medforf: Furnes, H
         UiB, Geologisk institutt
Medforf: Thorseth, I. H.
         UiB, Geologisk institutt
Medforf: Tumyr, O.
         UiB, Geologisk institutt
Medforf: Muehlenbachs, K
Tittel:  Microbial degradation of basaltic glass in the oceanic crust.
Tidskr:  NBS conference abstract
Red:     Brokstad, karl
År:      1998-10-17
Side(r): 1 s.
Konfer:  Norrwaegian Biochemical Soc. Multidisiplinary 30th aniversary.
Sted:    bergen
Emneord: basalt, weathering, microbial
Språk:   eng
PublID:  r99011574
LokalK:  s

72
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Torsvik, T.
         UiB, Institutt for mikrobiologi
Medforf: Bjelland, T
         UiB, Botanisk institutt
Medforf: Thorseth, I.H.
         UiB, Geologisk institutt
Medforf: Skaar, E
         UiB, Geofysisk institutt
Tittel:  Weathering of rock carvings in Vingen and Alta
Tidskr:  Abstract Acra II
Red:     Søborg, Hans Christian
År:      1998-09-02
Side(r): 1 s. s.
Konfer:  ACRA II.  The Alta cconference on rock art
Sted:    Alta
Emneord: Rock art, rock carvings, weathering
Språk:   eng
PublID:  r99011570
LokalK:  s

73
PublKat: A11 (Artikkel i internasjonalt vitenskapelig tidsskrift med referee)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Torsvik, Terje
         UiB, Institutt for mikrobiologi
Medforf: Furnes, Harald
         UiB, Geologisk institutt
Medforf: Muehlenbachs, Karlis
Medforf: Thorseth, Ingunn, H.,
         UiB, Geologisk institutt
Medforf: Tumyr, Ole
         UiB, Geologisk institutt
Tittel:  Evidence for microbial activity at the glass-alteration interface in o
         ceanic basalts
Tidskr:  Earth and planetary science letters
Red:     Albarede, F.
ISSN:    0012-821X
År:      1998
Volum:   162
Side(r): 165-176
Utgiver: Elsvier
Emneord: Costa Rica Rift, volcanic glass, basalts, microorganisms, alteration,
          geocemistry
SamEng:  Abstract A detailed microbiological and geochemical study related to
         the alteration of basaltic glass of pillow lavas from the oceanic cru
         st recove from Hole 896A on the Costa Rica Rift (penetrating 290 m in
         to the volcanic basement) has been carried out. A number of independe
         nt observations, pointing to the influence of microbes, may be summar
         ized as follows: 1) Alteration textures are reminiscent of microbes i
         n terms of form and shape. 2) Altered material contains appreciable a
         mounts of C, N and K, and the N/C ratios are comparable to those of n
         itrogen-starved bacteria. 3) Samples stained with a dye (DAPI) that b
         inds specifically to nucleic acids show the presence of DNA in the al
         tered glass. Further, staining with fluorescent labeled oligonucleoti
         de probes that hybridize specifically to 16S-ribosomal RNA of Bacteri
         a and Archaea demonstrate their presence in the altered part of the g
         lass. 4) Disseminated carbonate in the glassy margin of the majority
         of pillows shows d13C values, significantly lower than that of fresh
         basalt, also suggests biological activity. The majority of the sample
         s have d18O values indicating temperatures of 20 - 100oC, which is in
          the range of mesophilic and thermophilic micro-organisms.
Språk:   eng
PublID:  r99011549

74
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Torsvik, Terje
         UiB, Institutt for mikrobiologi
Medforf: Furnes, H.
         UiB, Geologisk institutt
Medforf: Muehlenbachs, K
Medforf: Thorseth, I. H.
         UiB, Geologisk institutt
Medforf: Tumyr, O.
         UiB, Geologisk institutt
Tittel:  Evidence for microbial activity at the glass-alteration interface in o
         ceanic basalt
Tidskr:  Abstract from Mikrobiolologisk vintermøte 98
Red:     Utkilen, Hans
År:      1998-02-19
Side(r): 1 s.
Konfer:  Mikrobiologisk vintermøte 98
Sted:    Voss
Emneord: Basaltic glass, microbial degradation
Språk:   eng
PublID:  r99011553
LokalK:  s

75
PublKat: C11 (Rapport utgitt av institusjon, forening, o.a.)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Torsvik, Terje
         UiB, Institutt for mikrobiologi
Tittel:  Mikroorganismer og deres betydning for forvitring av bergarter.
Serie:   Bergkunstrapporter fra UiB 1.  Sikring av bergkunst.
Red:     Lødøen, T. K.
Red:     Mandt, G.
Red:     Riisøen, K. H.
ISSN:    1500-3515
ISBN:    82-90273-64-9
År:      1998
Hefte:   1
Utgiver: Arkeologisk institutt, Bergen museum
Tilgang: Arkeologisk institutt, UiB, Håkon Sheteligs plass10, N-5007
Emneord: Helleristninger, forvitring, bevaring, mikroorganismer
SamEng:  This paper gives a general introduction to microorganisms and their w
         ay of life. Microorganisms constitute a very diverse group, both gene
         tically a physiologically. As part of their metabolic activity they a
         re involved in many of the great geochemical cycles. Bacteria and fun
         gi are the most important group of organisms that are involved in min
         eralizing organic mater. In their search for elements many of them al
         so take an active part in leaching processes in which minerals are di
         ssolved. The mechanisms used involve production of acids and bases th
         at change the environmental pH and accelerate the rate of mineral dis
         solution, redox processes that catalyze bioleaching of minerals and p
         roduction of organic acids agents and siderophores that specifically
         dissolve minerals by the process of metal complex formation.
Språk:   nor
PublID:  r99011550
LokalK:  N

76
PublKat: D04 (Uspesifisert foredrag med eller uten publisering (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Torsvik, Vigdis
         UiB, Institutt for mikrobiologi
Medforf: Daae, Frida Lise
         UiB, Institutt for mikrobiologi
Medforf: Øvreås, Lise
         UiB, Institutt for mikrobiologi
Tittel:  Diversity of soil microorganisms
År:      1998-01-14
Konfer:  NKJ/SNS Workshop on Soil Microbiology
Sted:    Uppsala, Sverige
Emneord: Microbiology, diversity, soil
Språk:   eng
PublID:  r99001307
LokalK:  As

77
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Torsvik, Vigdis
         UiB, Institutt for mikrobiologi
Medforf: Høj, Lone
         UiB, Institutt for mikrobiologi
Tittel:  Identification of bacteria and archaea from environmental samples by
         rRNA probing and DGGE analyses of rDNA.
År:      1998-03-20
Konfer:  Molecular Ecology Seminar
Sted:    Helsinki, Finland
Emneord: Archaea, diversity, molecular methods, enrichments
Språk:   eng
PublID:  r99001312
StatKat: f
LokalK:  As

78
PublKat: C12 (Alminnelig tilgjengelig kompendium)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Torsvik, Vigdis
         UiB, Institutt for mikrobiologi
Medforf: Goksøyr, Jostein
         UiB, Institutt for mikrobiologi
Tittel:  Kompendium i Molekylær mikrobiell økologi (BMP 318)
År:      1998
Side(r): 62 s.
Tilgang: Institutt for mikrobiologi, Vigdis Torsvik
Emneord: mikrobiell økologi, molekylære metoder, DNA, RNA
Språk:   nor
PublID:  r99001267
LokalK:  N

79
PublKat: D04 (Uspesifisert foredrag med eller uten publisering (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Torsvik, Vigdis
         UiB, Institutt for mikrobiologi
Tittel:  Molecular analyses of microbial communities in natural and perturbed e
         nvironments
År:      1998-02-13
Konfer:  Seminar i mikrobiell økologi
Sted:    Risø, Danmark
Emneord: microbial ecology, diversity, molecular methods
Språk:   eng
PublID:  r99001310
LokalK:  As

80
PublKat: A11 (Artikkel i internasjonalt vitenskapelig tidsskrift med referee)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Torsvik, Vigdis
         UiB, Institutt for mikrobiologi
Medforf: Daae, Frida Lise
         UiB, Institutt for mikrobiologi
Medforf: Sandaa, Ruth-Anne
         UiB, Institutt for mikrobiologi
Medforf: Øvreås, Lise
         UiB, Institutt for mikrobiologi
Tittel:  Novel techniques for analysing microbial diversity in natural and
         perturbed environments
Tidskr:  Journal of Biotechnology. Special Issue: Applied Molecular
         GeneticsEFB Working Party at the ECB8
Red:     Jakobsen, Kjetil
År:      1998
Volum:   64
Side(r): 53-62
Utgiver: Elsevier
Emneord: bacterial communities, diversity, DNA analysis, soil, sediments
SamEng:  Molecular techniques were applied for analysing the entire bacterial
         community, including both the cultivated and non-cultivated part of
         the community. DNA was extracted from sampl es of soils and
         sediments, and a combination of different molecular methods were used
         to investigate community structure and diversity in these
         environments. Reassociation of shearedand thermally denatured DNA in
         solution was used to measure the total genetical diversity. PCR- DGGE
         analysis of rRNA genes gave information about changes in the
         numerically dominating bacterial populations. Hybridisation with
         phylogenetic group specific probes, and sequencing provided
         information about the affiliation of the bacterial populations. Using
         DNA reassociation analysis we demonstrated that bacterial communities
         in pristine soil and sediments may contain more than 10 000 different
         bacterial types. The diversity of the total soil community was at
         least 200 times higher that the diversity of bacterial isolates from
         the same soil. This indicates that the culturing conditions select
         for a distinct subpopulation of the bacteria present in the
         environment. Molecular methods were applied to monitor the effects of
         perturbations due to antropogenic activities and pollution on
         microbial communities. O ur investigations show that agricultural
         management, fish farming and pollution may lead to profound changes
         in the community structure and a reduction in the bacterial
         diversity.
Språk:   eng
PublID:  r99001252
StatKat: f

81
PublKat: A11 (Artikkel i internasjonalt vitenskapelig tidsskrift med referee)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Øvreår, Lise
         UiB, Institutt for mikrobiologi
Medforf: Jensen, Sigmund
Medforf: Daae, Frida Lise
         UiB, Institutt for mikrobiologi
Medforf: Torsvik, Vigdis
         UiB, Institutt for mikrobiologi
Tittel:  Microbial community changes in a perturbed agricultural soil investiga
         ted by molecular and physiological approaches
Tidskr:  Applied and Environmental Microbiology
År:      1998
Volum:   64
Hefte:   7
Side(r): 2739-2742
Utgiver: American Society for Microbiology
Emneord: Microbial communities, activity, diversity, agricultural soil, physio
         logy, molecular approach
Språk:   eng
PublID:  r99001257

82
PublKat: C13 (Doktoravhandling)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Øvreås, Lise
         UiB, Institutt for mikrobiologi
Tittel:  Diversity of microbial populations and communities
ISBN:    82-7761-009-2
År:      1998-03-13
Side(r): 1 b.
Utgiver: Universitetet i Bergen
Tilgang: Institutt for mikrobiologi, Lise Øvreås
Emneord: microbiology, diversity, populations, communities
SamEng:  http://www.fou.uib.no/drgrad/1998/414001/
URL:     http://www.uib.no/info/dr_grad/98/mars/ovreas/ovreas.html
Språk:   eng
PublID:  r99001297
StatKat: a
LokalK:  N

83
PublKat: A11 (Artikkel i internasjonalt vitenskapelig tidsskrift med referee)
Instit:  Universitetet i Bergen, Det matematisk-naturvitenskapelige fakultet, Institutt for mikrobiologi
Forf:    Øvreås, Lise
         UiB, Institutt for mikrobiologi
Medforf: Torsvik, Vigdis
         UiB, Institutt for mikrobiologi
Tittel:  Microbial diversity and community structure in two different agricultu
         ral soil communities
Tidskr:  Microbial Ecology
År:      1998
Volum:   36
Side(r): 1-13
Utgiver: Springer-Verlag
Emneord: microbial,  diversity, community, structure, soil
Språk:   eng
PublID:  r99001263


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