Det odontologiske fakultet

Odontologisk institutt, Oral mikrobiologi

Produksjonsdato : 2001-02-02
Katalogtype: Fullformat.

1
PublKat: A11 (Artikkel i internasjonalt vitenskapelig tidsskrift med referee)
Instit:  Universitetet i Bergen, Det odontologiske fakultet, Odontologisk institutt, Oral mikrobiologi
Forf:    Ali, Raouf Wahab
Medforf: Bakken, Vidar
Medforf: Nilsen, Rune
Medforf: Skaug, Nils
Tittel:  Comaparative detection frequency of 6 putative periodontal pathogens i
         n Sudanese and Norwegian adult periodontitis patients.
Tidskr:  Journal of Periodontology
År:      1994
Volum:   65
Side(r): 1046-1052
Emneord: Periodontitis/pathogens; periodontitis/microbiology; race factors
SamNor:  TWENTY-FIVE SUDANESE AND 18 NORWEGIAN adult periodontitis patients we
         re selected to participate in this study. The purpose was to compare
         cultivati results of Porphyromonas gingivalis, Prevotella intermedia,
          Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum; and C
         apnocytophaga species as well as various enteric rods in both populat
         ions. In addition, DNA probe analysis was used to identify P. gingiva
         lis, P. intermedia, A. actinomycetemcomitans, F. nucleatum, and Bacte
         roides forsythus in the Sudanese patients and results were compared w
         ith those obtained by cultivation. The paper point technique was used
          to sample 99 sites in the Sudanese group (4 paper points/site) and 1
         19 sites in the Norwegian patients (3 paper points/site). In the Suda
         nese subjects, the fourth paper point was used for the DNA probe anal
         ysis. The chi-square test and the Wilcoxon signed rank test were used
          to test for statistically significant differences between Sudanese a
         nd Norwegian cultivation results as well as between cultivation and D
         NA results in the Sudanese group. Cultivation results indicated that
         the Sudanese subjects had significantly lower prevalence of P. gingiv
         alis, P. intermedia, and F. nucleatum (P < 0.01), significantly highe
         r prev alence of Capnocytophaga species (P < 0.05), and similar preva
         lence of A. actinomycetemcomitans. Almost all Sudanese subjects teste
         d positive for various enteric rods, while none of the Norwegians
           did so. The extent to which unrestricted use of antibiotics and
           transport media influenced the levels of enteric species is not
           known, however. In the Sudanese group, DNA results showed higher
            levels of P. gingivalis and P. intermedia (P < 0.01), and F.
         nucleatum (P < 0.05), while lower levels of A.      actinomycetemcomi
         tans (P < 0.05), as compared to cultivation results. (...)
Språk:   eng
PublID:  r96004319
StatKat: f

2
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det odontologiske fakultet, Odontologisk institutt, Oral mikrobiologi
Forf:    Ali, Raouf Wahab
Medforf: Bakken, Vidar
Medforf: Nilsen, Rune
Medforf: Skaug, Nils
Tittel:  Detection frequency of periopathogens in Sudanese and Norwegian period
         ontitis patients.
Tidskr:  Journal of Dental Research (Special Issue 1994 Abstracts)
År:      1994-03
Side(r): 254-
UtgSted: Seattle, USA
Konfer:  International Association for Dental Research
SamNor:  Twenty five Sudanese and 18 Norwegian adult periodontitis patients we
         re selected to participate in this study. The purpose was to compare
         cultivati results of Porphyromonas gingivalis, Prevotella intermedia,
          Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum, and C
         apnocytophaga species as well as various enteric rods in both populat
         ions. In addition, DNA probe analysis was used to identify P. gingiva
         lis, P. intermedia, A. actinomycetemcomitans, F. nucleatum, and Bacte
         roides forsythus in the Sudanese patients, and the results were compa
         red with those obtained by cultivation. Paper point technique was use
         d to sample 99 sites in the Sudanese group (4 paper points/site) and
         119 sites in the Norwegian patients (3paper points/site). In the Suda
         nese subjects, the fourth paper point was used for the DNA probe anal
         ysis. The chi-square test was used to test for significant difference
         s between Sudan ese and Norwegian cultivation results, as well as bet
         ween cultivation and DNA results in the Sudanese group. Cultivation r
         esults indicated that the Sudanese subjects, compared to theNorwegian
         s, had lower levels of P. gingivalis, P. intermedia, and F. nucleatum
          (p<0.01), but higher levels of Capnocytophaga species (p<0.05), and
         similar levels of A. actinomycetemcomitans. Almost all Sudanese subje
         cts tested positive for various enteric rods, while none of the Norwe
         gians did so. In the Sudanese group, DNA results indicated higher lev
         els of P. gi ngivalis, P. intermedia, and F. nucleatum and lower leve
         ls of A. actinomycetemcomitans, as compared to cultivation results. B
         . fo rsythus DNA probe detected the bacterium in 96 % of the Sudanese
          patients (57 % of the sites). Except for the high prevalence of ente
         ric rods in the Sudanese subjects, the present investigation demonstr
         ated that both populations harboured the species monitored in levels
         similar to those commonly perceived in the West.
Språk:   eng
PublID:  r96004313
StatKat: f

3
PublKat: A11 (Artikkel i internasjonalt vitenskapelig tidsskrift med referee)
Instit:  Universitetet i Bergen, Det odontologiske fakultet, Odontologisk institutt, Oral mikrobiologi
Forf:    Ali, Raouf Wahab
Medforf: Skaug, Nils
Medforf: Nilsen, Rune
Medforf: Bakken, Vidar
Tittel:  Microbial associations of 4 putative periodontal pathogens in Sudanese
          adult periodontitis patients determined by DNA probe analysis.
Tidskr:  Journal of Periodontology
År:      1994
Volum:   65
Side(r): 1053-1057
Emneord: Periodontitis/microbiology; periodontitis/pathogenesis; DNA probes
SamNor:  THE FEW PREVIOUS CULTIVATION STUDIES on the in vivo associations betw
         een various periodontal microbial species have shown several positive
          and negative associations. The present investigation utilized DNA pr
         obe analysis to examine possible in vivo associations between the per
         iodontal pathogens Porphyromonas gingivalis, Prevotella intermedia, F
         usobacterium nucleatum, and Bacteroides forsythus in subgingival plaq
         ue samples obtained from 25 Sudanese untreated adult periodontitis pa
         tients. The standard paper point techniq ue was used to sample 99 sit
         es with a mean probing depth og 6.8 mm (range 6.0 to 10.0). Microbial
          associations were determined by detecting the effect of the presence
         or absence of one species (effector) on the occurrence of the other 3
          species (target). The Wilcoxon signed rank test was used to examine
         variations in occurrence of each target bacteria in the presence or a
         bsence of the effector. In      addition, the  Spearman's rank correl
         ation test was used to      assess the  relationship between the leve
         l of each bacteria to      that of the other 3. Results showed bacter
         ial associations with      the  following effector-on-target effects:
          F. nucleatum (P<0.01      Wilcoxon; p<0.001 Spearman)
Språk:   eng
PublID:  r96004316
StatKat: f

4
PublKat: A11 (Artikkel i internasjonalt vitenskapelig tidsskrift med referee)
Instit:  Universitetet i Bergen, Det odontologiske fakultet, Odontologisk institutt, Oral mikrobiologi
Forf:    Ali, Raouf Wahab
         UiB-Odont: Laboratorium for oral mikrobiologi
Medforf: Lie, Tryggve
         UiB-Odont: Institutt for periodonti
Tittel:  Periodontal conditions as measured by CPITN in sudanese adolescents an
         d adults in two cities in Sudan
Tidskr:  The Saudi Dental Journal
År:      1994
Volum:   6
Hefte:   2
Side(r): 83-87
SamNor:  This study examines the prevalence and severity of periodontal condit
         ions and estimates the periodontal treatment needs by CPITN in groups
          of Sudanese subject. Study sample was grouped according to age and a
          total of 264 subjects were examined, 126 adolescents and 138 adults.
          Results indicated that the prevalence of periodontal disease among a
         dolescents was high, with 95.2% having pockets 4-5 mm and 4% having p
         ocket depths >/= 6mm. In the 35 to 44 years age-group, 71% of the sub
         jects had 4-5 mm pockets and 26% had pockets >/= 6 mm. Among th e 45
         to 64 years age-group, 64,5% had 4-5 mm pockets and 35.5% had pockets
          >/= 6 mm. All subjects in all age-groups needed oral hygiene instruc
         tions, and almost all required calculus removal for more than 5 sexta
         nts per subject. Complex (surgical) periodontal treatment was needed
         by 4% of adolescents to treat about 0.1 sextants per person, and by m
         ore than 30% of all adults to treat about 0.4 to 0.8 sextants per per
         son.
Språk:   eng
PublID:  r96004322
StatKat: f

5
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det odontologiske fakultet, Odontologisk institutt, Oral mikrobiologi
Forf:    Ali, Raouf Wahab
Medforf: Skaug, Nils
Medforf: Bakken, Vidar
Medforf: Nilsen, Rune
Tittel:  Survival of four putative periodontal pathogens and enteric bacteria i
         n the transport medium VMGA III
År:      1994-08
Side(r): 68-
UtgSted: Gøteborg, Sverige
Konfer:  77th Annual Meeting NOF. The Scandinavian Division of the Inter
         national Association for Dental Research
SamNor:  We recently used the «Viablity medium, Gøteborg, anaerobically prepar
         ed and sterilized» (VMGA III) to transport plaque samples from Sudan
         and Roman Norway. We experienced significant reduction in numbers of
         periopathogens and increase in enteric rods. The aim of this study wa
         s to determine the survivial in VMGA III of P. gingivalis (P.g.), P.
         intermedia (P.i),F. nucleatum (F.n.), A. actinomycetemcomitans (A.a.)
         , and enteric rods in pure cultures as well as in subgingival plaque
         samples. Laboratory strains of the 4 periopathogens and E. coli (E.c.
         ) were used to inoculate the medium vials for laboratory study. In ad
         dition, 30 subgingival plaque paper point samples were obtained from
         22 Romanian patients and stored in VMGA III. The content of each inoc
         ulated vial, in both studies, was divided into 3 portions, and plated
          at baseline, days 2 and 3. Differences from base line, in recovery o
         f each bacteria at days 2 and 3, were tested with the Wilcoxon signed
          rank test, while differences in the number of positive samples were
         tested by the chi square test. Any difference of p<0.05 wasconsidered
          as statistically significant (SS). Results of the clinical study sho
         w that the detection frequencies of all periopathogens decreased at d
         ay 2 (SS for F.n. and A.a.) and furt her decreased at day 3 (SS for F
         .n., A.a., and P.i.). The levels of these periopathogens in postive s
         amples also decreased at day 2 (SS for F.n.) and day 3 (SS fo r P.g.,
          A.a., P.i.,and F.n.). Enteric rods were not detected at baseline, wh
         ile they were significantly positive in 16.7 % and 23.3 % of the samp
         les at day 2 and day 3, respectively. The laboratory resu lts indicat
         e that among the 4 periopathogens tested, P.g. was the most stable, a
         lthough it decreased significantly at day 3. F.n. showed the poorest
         survival, followed by P.i .. E.c. increased significantly at day 2 an
         d further at day 3. [...] that P.g. and P.i.
Språk:   eng
PublID:  r96004306
StatKat: f

6
PublKat: A11 (Artikkel i internasjonalt vitenskapelig tidsskrift med referee)
Instit:  Universitetet i Bergen, Det odontologiske fakultet, Odontologisk institutt, Oral mikrobiologi
Forf:    Bolstad, Anne Isine
Medforf: Kleivdal, Hans
Medforf: Jensen, Harald B.
Tittel:  Similarities between Fusobacterium nucleatum and Bacteroides fragilis
         studied by two DNA probes derived from Fusobacterium nucleatum
Tidskr:  Scandinavian journal of dental research
År:      1994
Volum:   102
Side(r): 5-9
Emneord: Bacteroides fragilis; DNA probe; Fusobacterium nucleatum; immunoblott
         ing; polymerase chain reaction; SDS-PAGE
SamNor:  A polymerase chain reaction (PCR)-amplified oligonucleotide DNA probe
          corresponding to a Fusobacterium nucleatum Fev1 DNA region coding fo
         r a 40-kD major outer-membrane protein (OMP) and a randomly cloned 2.
         1 kb DNA probe were found to recognize DNA from the Gram-negative bac
         teria Fusobacterium nucleatum and Bacteroides fragilis on Southern bl
         ots and slot blots. The results indicate sequences similarity within
         the DNA fragments studied. Immunoblots tested with polyclonal antibod
         ies against whole cells of F. nucleatum revealed only weak antigen si
         milarity between these species.
Språk:   eng
PublID:  r96004303
StatKat: f

7
PublKat: A11 (Artikkel i internasjonalt vitenskapelig tidsskrift med referee)
Instit:  Universitetet i Bergen, Det odontologiske fakultet, Odontologisk institutt, Oral mikrobiologi
Forf:    Bolstad, Anne Isine
Tittel:  Sizing the Fusobacterium nucleatum genome by pulsed-field gel electrop
         horesis
Tidskr:  FEMS microbiology letters
År:      1994
Volum:   123
Side(r): 145-152
Emneord: Pulsed-field gel electrophoresis; Fusobacterium nucleatum; genome siz
         e; A+T rich genome; Gram-negative
SamNor:  The genome sizes of Fusobacterium nucleatum strains F1, F3, F6, ATCC
         10953, ATCC 25586 and Fev1 were determined by pulsed-field gel electr
         ophoresis (PFGE). The restriction enzymes SmaI, SacII, SalI and XhoI
         were found to generate a reasonable number of DNA fragments which cou
         ld be separated by PFGE in agarose gels. The apparent chromosomal len
         gths of the F. nucleat um strains were determined to be approximately
          2.4 million base pairs. This was within the size-range found by expe
         riments exploiting renaturation kinetics.
Språk:   eng
PublID:  r96004305
StatKat: f

8
PublKat: C13 (Doktoravhandling)
Instit:  Universitetet i Bergen, Det medisinske fakultet, Institutt for biokjemi og molekylærbiologi
Instit:  Universitetet i Bergen, Det odontologiske fakultet, Odontologisk institutt, Oral mikrobiologi
Instit:  Universitetet i Bergen, Det medisinske fakultet, Avdeling for mikrobiologi og immunologi, Gades institutt
Forf:    Bolstad, Anne Isine
         UiB-Odont: Laboratorium for oral mikrobiologi
Tittel:  The fomA gene encoding the 40-kDa porin monomer of Fusobacterium nucle
         atum
Serie:   dr.odont.
ISBN:    82-7249-149-4
År:      1994
Utgiver: Department of Biochemistry and Molecular Biology, University
         of Bergen
UtgSted: Bergen
Tilgang: Byttekontoret, Universitetsbiblioteket i Bergen
URL:     http://www.fou.uib.no/drgrad/1994/103001/
Språk:   eng
PublID:  r96004304
StatKat: a
LokalK:  KAT 4a

9
PublKat: A11 (Artikkel i internasjonalt vitenskapelig tidsskrift med referee)
Instit:  Universitetet i Bergen, Det odontologiske fakultet, Odontologisk institutt, Oral mikrobiologi
Forf:    Fredriksen, Åse
Medforf: Bakken, Vidar
Tittel:  Identification of Renibacterium salmoninarum surface proteins by radio
         iodin
År:      1994
Volum:   121
Side(r): 297-302
Emneord: Renibacterium salmoninarum; surface protein; radioiodination
SamNor:  Surface exposed proteins of Renibacterium salmoninarum were identifie
         d by radiolabelling whole bacterial cells with 125I, followed by SDS-
         PAGE and autoradiography. The most prominent bands had molecular mass
         es of approximately 57 kDa and 22 kDa; in addition, some less intensi
         vely labelled bands were detected. Polyclonal sera raised against the
          22 kDa protein di d not react with the 57 kDa protein. N-terminal am
         ino acid sequence analysis of the purified 22 kDa protein showed no s
         imilarity with the sequence of the 57 kDa protein.
Språk:   eng
PublID:  r96004300
StatKat: f

10
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det odontologiske fakultet, Odontologisk institutt, Oral mikrobiologi
Forf:    Ibsen, Margrethe
Medforf: Haneberg, Bjørn
Medforf: Bakken, Vidar
Medforf: Jonsson, Roland
Tittel:  Mucosal and systemic immune response to BCG in BALB/c mice.
Tidskr:  Scandinavian Society for Immunology, XXVth Annual Meeting a
         nd Xth Summer School
År:      1994-08
Side(r): 108-
UtgSted: Reykjavik, Island
SamNor:  The objective of this work was to investigate the humoral and cellula
         r immune response at mucosal surfaces, in serum and in lymphoid tissu
         e of experimental animals following BCG vaccination through oral and
         subcutanous routes. BALB/c mice were immunized by gastric, oral and s
         ubcutaneous delivery of Mycobacterium bovis BCG.The ELISPOT method wa
         s used to examine Ig and IFN-g production at the single cell level (s
         pot forming cells, sfc) with spleen B and T cells, respectively. T ce
         lls were stimulated for 72 hours with tuberculin, dead M. bovis BCG (
         BCG) and a heat shock protein (HSP65) from M. bovis BCG.ELISA was use
         d to determine levels of antigen specific Ig in serum and fecal sampl
         es. In the Ig ELISPOT assay we found considerably more IgG producing
         lymphocytes in subcutaneously immunized animals than in orally immuni
         zed animals and non-stimulated controls. After stimulation of T cells
         , cell cultures from gastric and subcutaneously immunized mice had hi
         gher numbers of IFN-g sfc than cell cultures from non-immunized co nt
         rols. In the serum ELISA, the antigen specific Igs were found to give
          higher OD in the subcutaneously immunized mice than in the controls.
          The mice immunized throughother routes showed higher content of anti
         gen specific IgM only. In the ELISA of fecal samples, there was no di
         fference between the various immunized groups and the controls. The s
         ubcutaneously immunized animals had a higher humoral response than th
         e other mice. The IFN-g production of antigen stimulated T cells, ind
         icates that mycobacteria l antigens induce murine cellular immune res
         ponse, when immunized through a gastric route as well as through a su
         bcutaneous route.
Språk:   eng
PublID:  r96004310
StatKat: f

11
PublKat: A11 (Artikkel i internasjonalt vitenskapelig tidsskrift med referee)
Instit:  Universitetet i Bergen, Det odontologiske fakultet, Odontologisk institutt, Oral mikrobiologi
Forf:    Løkensgard, Ingunn
Medforf: Bakken, Vidar
Medforf: Schenck, Karl
Tittel:  Heat shock response in Actinobacillus actinomycetemcomitans
År:      1994
Volum:   8
Side(r): 321-328
Emneord: Actinobacillus actinomycetemcomitans; periodontitis, Heat shock prote
         ins; stress proteins
SamNor:  The heat shock response in Actinobacillus actinomycetemcomitans, a ca
         pnophilic Gram-negative bacterial species that is implicated in the d
         evelopment of certain forms of periodontitis, was characterized. Diff
         erent strains of A. actinomycetemcomitans were grown at 37, 42 and 48
          oC in the presence of 35S-methionine. The bacterial cells were lysed
         , run on SDS-PAGE and subsequently blotted on nitrocellulose paper. A
         fter autoradiography of the blots, several protein bands from the cul
         tures at 42 oC showed an increased intensity; major bands were observ
         ed at 90, 70 and 60 kDa, but increased protein synthesis was also det
         ected at 54, 28 and 17 kDa. Nitrocellulose blots were also incubated
         witha panel of monoclonal and polyclonal antibodies directed to epito
         pes on different heat shock proteins. Strong reactivity was found wit
         h several antibodies at the positi on corresponding to a molecular ma
         ss of 60 kDa. The protein is probably the GroEL homologue in A. actin
         omyctemcomitans, a member of the ½common bacterial antigen+ family.
Språk:   eng
PublID:  r96004299
StatKat: f

12
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det odontologiske fakultet, Odontologisk institutt, Oral mikrobiologi
Forf:    Nunes, Ivana
Medforf: Bakken, Vidar
Medforf: Jonsson, Roland
Tittel:  Fas antigen and HLA-DR expression on epithelial and inflammatory cells
          in gingival tissues.
År:      1994-08
Side(r): 109-
UtgSted: Reykjavik, Island
Konfer:  Scandinavian Society for Immunolgy, XXVth Annual Meeting and Xt
         h Summer School
SamNor:  Fas is an antigen which belongs to the tumor necrosis factor/nerve gr
         owth factor receptor family and mediates apoptosis. HLA-DR is involve
         d in antigen handling and presentation during stimulation of T lympho
         cytes. Fas and HLA-DR are both signaling receptor molecules found on
         the surface of a number of cells, e.g. lymphocytes. Our aim in this s
         tudy was to evaluate the tissue distribution of Fas and HLA-DR in gin
         gival tissues of patients with adult periodontitis (AP) and clinicall
         y healthy gingiva (HG). Immunohistochemical analysis of cryostat sect
         ions was performed on biopsies from 8 patients with AP and 7 with HG.
          Biopsies were incubated with monoclonal antibodies against Fas and H
         LA-DR. Fas expression was observed on mononuclear cells (MNC) in the
         inflammatory infiltrate, endothelial cells (EC) and also on epithelia
         l cells as keratinocytes (KC), den dritic cells (DC) of both oral gin
         gival epithelium (OGE) and pocket epithelium (PE) in AP. However, in
         HG we found Fas expressed in 3 of 7 biopsies, on MNC, EC and on few K
         C of basal cell layer and papillary projections, the other 4 biopsies
          were totally negative. HLA-DR, in AP, was expressed on MNC, DC, EC a
         nd on KC, while in HG the expression was restricted to MNC, and DC bu
         t not on KC in a ll tissue samples analysed. Our observations indicat
         es that HLA-DR+ cells were more frequent than Fas both in AP and HG,
         except for the epithelial expression in the HG where we observed Fas
         expressed on few KC, but not HLA-DR. The variation found on the expre
         ssion of Fas and HLA-DR, both in disease and normal gingiva indicates
          that these antigens may play a role in the development of periodonta
         l disease and may also reflect disease activity. The expression of HL
         A-DR and Fas on KC, may explain not only the normal tissue turn over,
          but maybe also the proliferation of the pocket epithelium.
Språk:   eng
PublID:  r96004309
StatKat: f

13
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det odontologiske fakultet, Odontologisk institutt, Oral mikrobiologi
Forf:    Skaug, Nils
Medforf: Ali, Raouf Wahab
Medforf: Nilsen, Rune
Medforf: Bakken, Vidar
Tittel:  Microbial associations of periodontal pathogens in subgingival plaque
         from periodontitis patients.
Tidskr:  Journal of Dental Research (Special Issue 1994 Abstracts)
År:      1994-03
Side(r): 253-
UtgSted: Seattle, USA
Konfer:  International Association for Dental Research
SamNor:  The few published cultivation studies of in vivo associations between
          various periodontal microbial species have shown several positive, n
         egative a neutral associations. The present investigation utilised DN
         A probe analysis to examine possible in vivo associations between the
          periodontal pathogens Porphyromonas gingivalis, Prevotella intermedi
         a, Fusobacterium nucleatum, and Bacteroides forsythus in subgingival
         plaque samples obtained from 25 Sudanese adult periodontitis patients
         . The standard paper point technique was used to sample 99 sites with
          a mean pocket depth of 6.8 mm (range 6.010.0). DNA probe analysis wa
         s used to enumerate the 4 species. Microbial associations were define
         d by detecting the effect of the presence or absence of one species (
         effector) on the occurrence of the other 3 species (targets). The Wil
         coxon signed rank test was used to examine variations in occurence of
          each target ba cteria in the presence or absence of the effector. In
          addition, the Spearman's rank correlation test was used to assess th
         e relationship between the level of each bacteria to that ofthe other
          3 ones. Results showed that the presence of F. nucleatum had the gre
         atest impact on the colonisation of the other 3 species; they were id
         entified in significantly more patie nts and sites and in higher prop
         ortions when present together with F. nucleatum than without (p<0.01)
         . The same trend was shown for P. gingivalis and B. forsythus, while
         P. inte rmedia had the least effect on the colonisation of the other
         3 species. The study demonstrated positive associations between the 4
          species investigated. The most striking finding was the effect exert
         ed by F. nucleatum on the colonisation of P. intermedia, which was ne
         ver detected in a site unless F. nucleatum was present at the same si
         te. Th is study was supported by RMF/NAVF grant No. 334.93/012, and G
         ade's legat.
Språk:   eng
PublID:  r96004312
StatKat: f

14
PublKat: C14 (Magisteravhandling)
Instit:  Universitetet i Bergen, Det odontologiske fakultet, Odontologisk institutt, Oral mikrobiologi
Forf:    Velcescu, Cristina
         UiB-Odont: Laboratorium for oral mikrobiologi
Tittel:  Bacteria and host responses in periodontal diseases with special refer
         ence to three key pathogens in Romanian adult patients
ISBN:    82-7249-152-4
År:      1994-12
Språk:   eng
PublID:  r96004307
StatKat: f

15
PublKat: D03 (Foredrag/poster ved vit. konf. med publisert abstract (t.o.m. 1998))
Instit:  Universitetet i Bergen, Det odontologiske fakultet, Odontologisk institutt, Oral mikrobiologi
Forf:    Velcescu, Cristina
Medforf: Skaug, Nils
Medforf: Bakken, Vidar
Medforf: Matre, Roald
Tittel:  Detection frequency of three key periopathogens in subgingival plaque
         and homologous serum IgG Ab in Romanian adult periodontitis patients
År:      1994-08
Side(r): 68-
UtgSted: Gøteborg, Sverige
Konfer:  77th Annual Meeting of NOF. The Scandinavian Division of the  I
         nternational Association for Dental Research
SamNor:  It has been reported that periodontal pockets ³ 6 mm deep are more li
         kely than shallow ones to harbor the key periopathogens Porphyromonas
          gingivalis, (P.g.), Actinobacillus actinomycetemcomitans (A.a.), and
          Bacteroides forsythus (B.f) at      levels of 104 - 105 as an indica
         tion of active disease. The aims     of this cross-sectional study we
         re (i) to see if deep and shallow      pockets differed significantly
          with respest to detection     frequency of P.g., A.a., and B.f., and
          (ii) to quantify serum IgG      antibody specific for P.g., A.a., an
         d B.f., respectively. Paper      point subgingival plaque samples and
          venous blood were obtained      from 17 Romanian adult periodontitis
          patients, 12 with advanced      and 5 with moderate disease. The key
          bacteria were detected by      DNA probe analysis (AffirmÖ DP, Micro
         Probe Corp. USA) at levels     of 104 (A.a.) and 105 (P.g. and B.f.)
         per plaque sample. The sera      were screened for IgG to whole cell
         preparations of clinical      isolates and laboratory strains using E
         LISA. Sera from nine      children aged 1-3 years served as negative
         serum controls.      Negative ELISA titer level was defined as mean
         _ a2 S.D. The      detection frequency of P.g. was significantly high
         er (p<0.01)      than that of A.a. and B.f. in both patient groups. T
         here was no      statistically significant difference between detecti
         on frequency      of the key bacteria in deep and shallow pockets in
         the two      groups. A strong correlation (R
Språk:   eng
PublID:  r96004311
StatKat: f


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